Overview

  • Product nameAnti-RGS9 antibody
    See all RGS9 primary antibodies
  • Description
    Rabbit polyclonal to RGS9
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rat, Horse, Guinea pig, Cat
  • Immunogen

    Synthetic peptide corresponding to a region within internal sequence amino acids 624-673 of Human RGS9 (NP_003826)

  • Positive control
    • Human fetal spleen lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab97702 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 77 kDa. Optimal concentration of antibody depends the protein level in a given sample. Our recommended concentrations are the starting point for general use. Customers are advised have to do a series of titration tests to determine optimal concentration. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionInhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form. Binds to G(t)-alpha. Involved in phototransduction; key element in the recovery phase of visual transduction.
  • Tissue specificityHighly expressed in the caudate and putamen, lower levels found in the hypothalamus and nucleus accumbens and very low levels in cerebellum. Not expressed in globus pallidus or cingulate cortex. Isoform 2 is expressed predominantly in pineal gland and retina. Isoform 3 is expressed in retina (abundant in photoreceptors).
  • Involvement in diseaseDefects in RGS9 are a cause of prolonged electroretinal response suppression (PERRS) [MIM:608415]; also known as bradyopsia. PERRS is characterized by difficulty adjusting to sudden changes in luminance levels mediated by cones.
  • Sequence similaritiesContains 1 DEP domain.
    Contains 1 G protein gamma domain.
    Contains 1 RGS domain.
  • DomainIn photoreceptor cells the DEP domain is essential for targeting RGS9 to the outer rod segments.
  • Post-translational
    modifications
    Retinal isoform 3 is light-dependent phosphorylated at 'Ser-478'. Phosphorylation is decreased by light exposition.
  • Cellular localizationMembrane. Isoform 3 is targeted to the membrane via its interaction with RGS9BP.
  • Information by UniProt
  • Database links
  • Alternative names
    • PERRS antibody
    • R9AP antibody
    • Regulator of G protein signalling 9 antibody
    • Regulator of G-protein signaling 9 antibody
    • RGS9 antibody
    • RGS9_HUMAN antibody
    • RGS9L antibody
    see all

Anti-RGS9 antibody images

  • Anti-RGS9 antibody (ab97702) at 1 µg/ml (in 5% skim milk / PBS buffer) + human fetal spleen lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 77 kDa

References for Anti-RGS9 antibody (ab97702)

ab97702 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"