Overview

  • Product name
    Anti-RGS9 antibody [EPR2873]
    See all RGS9 primary antibodies
  • Description
    Rabbit monoclonal [EPR2873] to RGS9
  • Tested applications
    Suitable for: WB, Flow Cyt, ICC/IFmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within RGS9. The exact sequence is proprietary.

  • Positive control
    • WB: Y79 and PC12 cell lysates and human and mouse spleen tissue lysates. ICC/IF: Neuro-2a cells. Flow Cyt: PC12 and Y79 cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 59% PBS, 0.05% BSA
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    EPR2873
  • Isotype
    IgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab108975 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 55 kDa (predicted molecular weight: 77 kDa).
Flow Cyt 1/30 - 1/60.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF 1/50 - 1/100.
  • Application notes
    Is unsuitable for IHC-P or IP.
  • Target

    • Function
      Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form. Binds to G(t)-alpha. Involved in phototransduction; key element in the recovery phase of visual transduction.
    • Tissue specificity
      Highly expressed in the caudate and putamen, lower levels found in the hypothalamus and nucleus accumbens and very low levels in cerebellum. Not expressed in globus pallidus or cingulate cortex. Isoform 2 is expressed predominantly in pineal gland and retina. Isoform 3 is expressed in retina (abundant in photoreceptors).
    • Involvement in disease
      Defects in RGS9 are a cause of prolonged electroretinal response suppression (PERRS) [MIM:608415]; also known as bradyopsia. PERRS is characterized by difficulty adjusting to sudden changes in luminance levels mediated by cones.
    • Sequence similarities
      Contains 1 DEP domain.
      Contains 1 G protein gamma domain.
      Contains 1 RGS domain.
    • Domain
      In photoreceptor cells the DEP domain is essential for targeting RGS9 to the outer rod segments.
    • Post-translational
      modifications
      Retinal isoform 3 is light-dependent phosphorylated at 'Ser-478'. Phosphorylation is decreased by light exposition.
    • Cellular localization
      Membrane. Isoform 3 is targeted to the membrane via its interaction with RGS9BP.
    • Information by UniProt
    • Database links
    • Alternative names
      • PERRS antibody
      • R9AP antibody
      • Regulator of G protein signalling 9 antibody
      • Regulator of G-protein signaling 9 antibody
      • RGS9 antibody
      • RGS9_HUMAN antibody
      • RGS9L antibody
      see all

    Anti-RGS9 antibody [EPR2873] images

    • All lanes : Anti-RGS9 antibody [EPR2873] (ab108975) at 1/1000 dilution (purified)

      Lane 1 : Y79 whole cell lysate
      Lane 2 : Mouse spleen tissue lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG at 1/1000 dilution

      Predicted band size : 77 kDa
      Observed band size : 55 kDa (why is the actual band size different from the predicted?)
      Blocking and dilution buffer: 5% NFDM/TBST.
    • Anti-RGS9 antibody [EPR2873] (ab108975) at 1/1000 dilution (purified) + PC-12 whole cell lysate at 10 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size : 77 kDa
      Observed band size : 55 kDa (why is the actual band size different from the predicted?)
      Blocking and dilution buffer: 5% NFDM/TBST.
    • Immunocytochemistry/Immunofluorescence analysis of Neuro-2a cells labelling RGS9 with purified ab108975 at a dilution of 1/100. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000) were also used.

      Control 1: primary antibody (1/100) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000).

      Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000).

    • Flow Cytometry analysis of Y79 cells labelling RGS9 with purified ab108975 at a dilution of 1/60 (red). Cells were fixed with 4% paraformaldehyde. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.

    • All lanes : Anti-RGS9 antibody [EPR2873] (ab108975) at 1/1000 dilution (unpurified)

      Lane 1 : PC12 lysates
      Lane 2 : Human spleen lysates

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

      Predicted band size : 77 kDa
      Observed band size : 55 kDa (why is the actual band size different from the predicted?)
      Note: Predicted molecular weights for UniProt ID O75916 isoforms 1 to 4 are ~77, 55, 57 and 50 kDa respectively. Therefore, the observed WB band may correspond to isoform 2, 3 or 4.
    • Flow cytometric analysis of permeabilized PC12 cells labelling RGS9 with unpurified ab108975 (red) at a dilution of 1/30 or a rabbit IgG (negative) (green).

    References for Anti-RGS9 antibody [EPR2873] (ab108975)

    This product has been referenced in:
    • Vogt A  et al. Isotope coded protein labeling coupled immunoprecipitation (ICPL-IP): a novel approach for quantitative protein complex analysis from native tissue. Mol Cell Proteomics 12:1395-406 (2013). Read more (PubMed: 23268931) »

    See 1 Publication for this product

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    There are currently no Abreviews or Questions for ab108975.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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