The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 97 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Use at an assay dependent concentration.
ELISA titre using peptide based assay: 1/62500.
Use at an assay dependent concentration. PubMed: 24648344
RelevanceRHBDF1 is a seven-transmembrane protein with a long N-terminal cytoplasmic extension that comprises half of the polypeptide sequence, and is found in the endoplasmic reticulum and Golgi, but not on the cell surface. RHBDF1 has a pivotal role in sustaining growth signals in epithelial cancer cells and thus may serve as a therapeutic target for treating epithelial cancers.
Cellular localizationEndoplasmic reticulum and Golgi Apparatus
epidermal growth factor receptor related sequence antibody
FLJ 2235 antibody
FLJ 22357 antibody
gene 89 antibody
gene 90 antibody
h Dist 1 antibody
hDist 1 antibody
RHBDF 1 antibody
rhomboid 5 homolog 1 (Drosophila) antibody
Rhomboid 5 homolog 1 antibody
rhomboid family 1 antibody
Anti-RHBDF1 antibody images
Western blot - RHBDF1 antibody (ab81342)
Anti-RHBDF1 antibody (ab81342) at 1 µg/ml (in 5% skim milk / PBS buffer) + 721_B cell lysate
Secondary HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 97 kDa
References for Anti-RHBDF1 antibody (ab81342)
This product has been referenced in:
Zhou Z et al. Human rhomboid family-1 suppresses oxygen-independent degradation of hypoxia-inducible factor-1a in breast cancer. Cancer Res74:2719-30 (2014).
Read more (PubMed: 24648344) »