Overview

  • Product nameAnti-RHBDF2 antibody
  • Description
    Rabbit polyclonal to RHBDF2
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH, corresponding to a region within N terminal amino acids 79-109 of Human RHBDF2 (NP_001005498.2, NP_078875.4).

  • Positive control
    • CEM cell line lysates.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
  • Storage bufferPreservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesab116139 is purified through a protein A column, followed by peptide affinity purification.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab116139 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 97 kDa.Can be blocked with RHBDF2 peptide (ab216174).

Target

  • FunctionRhomboid protease-like protein which has no protease activity but regulates the secretion of several ligands of the epidermal growth factor receptor. Indirectly activates the epidermal growth factor receptor signaling pathway and may thereby regulate sleep, cell survival, proliferation and migration.
  • Sequence similaritiesBelongs to the peptidase S54 family.
  • Cellular localizationEndoplasmic reticulum membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Inactive rhomboid protein 2 antibody
    • iRhom2 antibody
    • Rhbdf2 antibody
    • RHBDL5 antibody
    • RHBDL6 antibody
    • RHDF2_HUMAN antibody
    • Rhomboid 5 homolog 2 (Drosophila) antibody
    • Rhomboid 5 homolog 2 antibody
    • Rhomboid family member 2 antibody
    • Rhomboid veinlet-like protein 5 antibody
    • Rhomboid veinlet-like protein 6 antibody
    • TOC antibody
    • TOCG antibody
    • Tylosis with oesophageal cancer antibody
    see all

Anti-RHBDF2 antibody images

  • Anti-RHBDF2 antibody (ab116139) at 1/100 dilution + CEM cell line lysate at 35 µg

    Predicted band size : 97 kDa

References for Anti-RHBDF2 antibody (ab116139)

ab116139 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (keratinocyte)
Specification keratinocyte
Fixative Formaldehyde
Permeabilization Yes - 0.6% Triton-X in PBS
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 4°C
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Submitted Aug 03 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"