Anti-RhD antibody (ab222792)
Key features and details
- Rabbit polyclonal to RhD
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-RhD antibody
See all RhD primary antibodies -
Description
Rabbit polyclonal to RhD -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment corresponding to Human RhD aa 32-76.
Database link: Q02161 -
Positive control
- WB: Mouse liver lysate. IHC-P: Human kidney tissue. ICC/IF: A549 (human lung carcinoma cell line) cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
>95% pure -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab222792 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/20 - 1/200.
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ICC/IF |
1/50 - 1/200.
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WB |
1/2000 - 1/5000. Predicted molecular weight: 26 kDa.
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Notes |
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IHC-P
1/20 - 1/200. |
ICC/IF
1/50 - 1/200. |
WB
1/2000 - 1/5000. Predicted molecular weight: 26 kDa. |
Target
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Function
May be part of an oligomeric complex which is likely to have a transport or channel function in the erythrocyte membrane. -
Tissue specificity
Restricted to tissues or cell lines expressing erythroid characters. -
Sequence similarities
Belongs to the ammonium transporter (TC 2.A.49) family. Rh subfamily. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 6007 Human
- Entrez Gene: 19746 Mouse
- Omim: 111680 Human
- SwissProt: Q02161 Human
- SwissProt: Q8CF94 Mouse
- Unigene: 449968 Human
- Unigene: 195461 Mouse
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Alternative names
- Blood group Rh(D) polypeptide antibody
- Blood group--Rhesus system D polypeptide antibody
- CD240D antibody
see all
Images
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Anti-RhD antibody (ab222792) at 1/2000 dilution + mouse liver tissue lysate
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 26 kDa -
Paraffin embedded human kidney tissue stained for RhD with ab222792 at a 1/100 dilution.
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A549 (human lung carcinoma cell line) cells stained for RhD (green) using ab222792 at a 1/100 dilution in ICC/IF. Secondary: Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab222792 has not yet been referenced specifically in any publications.