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Storage instructions Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage buffer pH: 7.40 Preservative: 0.02% Sodium azide Constituent: PBS Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
Purity Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 18 kDa (predicted molecular weight: 20 kDa).
Function Stimulates the phosphorylation of S6K1 and EIF4EBP1 through activation of mTORC1 signaling. Activates the protein kinase activity of mTORC1. Has low intrinsic GTPase activity.
Tissue specificity Ubiquitous. Highest levels observed in skeletal and cardiac muscle.
Sequence similarities Belongs to the small GTPase superfamily. Rheb family.
Post-translational modifications Farnesylation is important for efficiently activating mTORC1-mediated signaling.
Cellular localization Cell membrane.
Information by UniProt
Ras homolog enriched in brain 2, formerly antibody
GTP binding protein Rheb antibody
GTP-binding protein Rheb antibody
Anti-RHEB antibody images
Western blot - Anti-RHEB antibody (ab109914)
All lanes : Anti-RHEB antibody (ab109914) at 1 µg/ml Lane 1 : Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate Lane 2 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate Lane 3 : EGF-Stimulated A431 Whole Cell Lysate Lane 4 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate Lysates/proteins at 10 µg per lane. Secondary Goat Anti-Rabbit IgG H&L (HRP) ( ab97051) at 1/10000 dilution Developed using the ECL technique Performed under reducing conditions. Predicted band size : 20 kDa Observed band size : 18 kDa ( why is the actual band size different from the predicted?) Additional bands at : 42 kDa,49 kDa,95 kDa. We are unsure as to the identity of these extra bands. Exposure time : 20 minutes
References for Anti-RHEB antibody (ab109914)
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"