Overview

  • Product name
  • Description
    Rabbit polyclonal to RHEB
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rat, Rabbit, Horse, Cow, Pig, Chimpanzee, Macaque monkey, Gorilla
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 100 to the C-terminus of Human RHEB.

  • Positive control
    • This antibody gave a positive signal in the following whole cell lysates: Raji; SHSY5Y; EGF treated A431; Jurkat.

Properties

Applications

Our Abpromise guarantee covers the use of ab109914 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 18 kDa (predicted molecular weight: 20 kDa).

Target

  • Function
    Stimulates the phosphorylation of S6K1 and EIF4EBP1 through activation of mTORC1 signaling. Activates the protein kinase activity of mTORC1. Has low intrinsic GTPase activity.
  • Tissue specificity
    Ubiquitous. Highest levels observed in skeletal and cardiac muscle.
  • Sequence similarities
    Belongs to the small GTPase superfamily. Rheb family.
  • Post-translational
    modifications
    Farnesylation is important for efficiently activating mTORC1-mediated signaling.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Ras homolog enriched in brain 2, formerly antibody
    • GTP binding protein Rheb antibody
    • GTP-binding protein Rheb antibody
    • MGC111559 antibody
    • Ras homolog enriched in brain 2 antibody
    • Ras homolog enriched in brain antibody
    • RHEB 2 antibody
    • Rheb antibody
    • RHEB_HUMAN antibody
    • RHEB2 antibody
    • RHEB2, formerly antibody
    see all

Images

  • All lanes : Anti-RHEB antibody (ab109914) at 1 µg/ml

    Lane 1 : Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate
    Lane 2 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate
    Lane 3 : EGF-Stimulated A431 Whole Cell Lysate
    Lane 4 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 20 kDa
    Observed band size : 18 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 42 kDa,49 kDa,95 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes

References

ab109914 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab109914.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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