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Rabbit polyclonal to RHEB
Predicted to work with:
Rat, Rabbit, Horse, Cow, Pig, Chimpanzee, Macaque monkey, Gorilla
Synthetic peptide conjugated to KLH derived from within residues 100 to the C-terminus of Human RHEB.
This antibody gave a positive signal in the following whole cell lysates: Raji; SHSY5Y; EGF treated A431; Jurkat.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium azide Constituent: PBS Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 18 kDa (predicted molecular weight: 20 kDa).
Stimulates the phosphorylation of S6K1 and EIF4EBP1 through activation of mTORC1 signaling. Activates the protein kinase activity of mTORC1. Has low intrinsic GTPase activity.
Ubiquitous. Highest levels observed in skeletal and cardiac muscle.
Belongs to the small GTPase superfamily. Rheb family.
Farnesylation is important for efficiently activating mTORC1-mediated signaling.
Information by UniProt
Ras homolog enriched in brain 2, formerly antibody
GTP binding protein Rheb antibody
GTP-binding protein Rheb antibody
Western blot - Anti-RHEB antibody (ab109914)
All lanes :
Anti-RHEB antibody (ab109914) at 1 µg/ml
Lane 1 :
Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate
Lane 2 :
SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate
Lane 3 :
EGF-Stimulated A431 Whole Cell Lysate
Lane 4 :
Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Goat Anti-Rabbit IgG H&L (HRP) (
) at 1/10000 dilution
Developed using the ECL technique
Performed under reducing conditions.
Predicted band size :
Observed band size :
18 kDa (
why is the actual band size different from the predicted?
Additional bands at :
42 kDa,49 kDa,95 kDa. We are unsure as to the identity of these extra bands.
Exposure time :
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"