Overview

  • Product nameAnti-RHEB antibody
    See all RHEB primary antibodies
  • Description
    Rabbit polyclonal to RHEB
  • Specificityab25873 recognises Rheb (Ras homolog enriched in brain).
  • Tested applicationsSuitable for: IHC-P, ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Drosophila melanogaster
  • Immunogen

    A 15 amino acid peptide from the middle region of Human Rheb.

  • Positive control
    • Mouse brain cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab25873 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 2 µg/ml.
ICC/IF Use a concentration of 20 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 20 kDa.Can be blocked with Active human RHEB peptide (ab39861).

Target

  • FunctionStimulates the phosphorylation of S6K1 and EIF4EBP1 through activation of mTORC1 signaling. Activates the protein kinase activity of mTORC1. Has low intrinsic GTPase activity.
  • Tissue specificityUbiquitous. Highest levels observed in skeletal and cardiac muscle.
  • Sequence similaritiesBelongs to the small GTPase superfamily. Rheb family.
  • Post-translational
    modifications
    Farnesylation is important for efficiently activating mTORC1-mediated signaling.
  • Cellular localizationCell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Ras homolog enriched in brain 2, formerly antibody
    • GTP binding protein Rheb antibody
    • GTP-binding protein Rheb antibody
    • MGC111559 antibody
    • Ras homolog enriched in brain 2 antibody
    • Ras homolog enriched in brain antibody
    • RHEB 2 antibody
    • Rheb antibody
    • RHEB_HUMAN antibody
    • RHEB2 antibody
    • RHEB2, formerly antibody
    see all

Anti-RHEB antibody images



  • Predicted band size : 20 kDa
    Observed band size : 17 kDa (why is the actual band size different from the predicted?)
  • ab25873 at 2µg/ml staining RHEB in mouse brain tissue by IHC

References for Anti-RHEB antibody (ab25873)

This product has been referenced in:
  • Luo J  et al. Insulin/IGF-regulated size scaling of neuroendocrine cells expressing the bHLH transcription factor Dimmed in Drosophila. PLoS Genet 9:e1004052 (2013). ICC/IF ; Drosophila melanogaster . Read more (PubMed: 24385933) »
  • Jia CH  et al. IKK-ß mediates hydrogen peroxide induced cell death through p85 S6K1. Cell Death Differ : (2012). Read more (PubMed: 22955948) »

See all 4 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (mammary, MDA-MB-435)
Loading amount 20 µg
Specification mammary, MDA-MB-435
Gel Running Conditions Reduced Denaturing (15)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Verified customer

Submitted Mar 05 2009

Thank you for your enquiry. The peptide sequence used is proprietary, but 12/14 residues are identical between the human Rheb and drosophila CG1081. ************ * (Residues 12 and 13 have no homology.) I performed a BLAST as well for the drosophil...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"