Overview

  • Product nameAnti-RHEB antibody
    See all RHEB primary antibodies
  • Description
    Rabbit polyclonal to RHEB
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide, corresponding to 14 amino acids at the N terminus of Human RHEB.

  • Positive control
    • Rat heart cell lysate can be used as positive control.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab25976 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notesWB: Use at a concentration of 2 - 4 µg/ml. Detects a band of approximately 20 kDa (predicted molecular weight: 20 kDa). Can be blocked with RHEB peptide (ab39862).
    Post-translationally modified Rheb is sometimes observed at higher molecular weight.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionStimulates the phosphorylation of S6K1 and EIF4EBP1 through activation of mTORC1 signaling. Activates the protein kinase activity of mTORC1. Has low intrinsic GTPase activity.
    • Tissue specificityUbiquitous. Highest levels observed in skeletal and cardiac muscle.
    • Sequence similaritiesBelongs to the small GTPase superfamily. Rheb family.
    • Post-translational
      modifications
      Farnesylation is important for efficiently activating mTORC1-mediated signaling.
    • Cellular localizationCell membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • Ras homolog enriched in brain 2, formerly antibody
      • GTP binding protein Rheb antibody
      • GTP-binding protein Rheb antibody
      • MGC111559 antibody
      • Ras homolog enriched in brain 2 antibody
      • Ras homolog enriched in brain antibody
      • RHEB 2 antibody
      • Rheb antibody
      • RHEB_HUMAN antibody
      • RHEB2 antibody
      • RHEB2, formerly antibody
      see all

    Anti-RHEB antibody images

    • Lane 1 : Anti-RHEB antibody (ab25976) at 2 µg/ml
      Lane 2 : Anti-RHEB antibody (ab25976) at 4 µg/ml

      Lane 1 : Rat heart cell lysate
      Lane 2 : Rat heart cell lysate


      Predicted band size : 20 kDa
      Observed band size : 20 kDa

    References for Anti-RHEB antibody (ab25976)

    ab25976 has not yet been referenced specifically in any publications.

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    There are currently no Abreviews or Questions for ab25976.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"