Overview

  • Product nameAnti-RHEB antibody
    See all RHEB primary antibodies
  • Description
    Mouse monoclonal to RHEB
  • Tested applicationsSuitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant full length Human RHEB protein (AAH16155, 1 a.a. ~ 185 a.a) with tag.

  • Positive control
    • Jurkat cell lysate; Recombinant tagged full length human RHEB.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab76629 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent dilution. Detection limit for recombinant tagged RHEB is approximately 3ng/ml if used as a capture antibody.
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 20 kDa.

Target

  • FunctionStimulates the phosphorylation of S6K1 and EIF4EBP1 through activation of mTORC1 signaling. Activates the protein kinase activity of mTORC1. Has low intrinsic GTPase activity.
  • Tissue specificityUbiquitous. Highest levels observed in skeletal and cardiac muscle.
  • Sequence similaritiesBelongs to the small GTPase superfamily. Rheb family.
  • Post-translational
    modifications
    Farnesylation is important for efficiently activating mTORC1-mediated signaling.
  • Cellular localizationCell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Ras homolog enriched in brain 2, formerly antibody
    • GTP binding protein Rheb antibody
    • GTP-binding protein Rheb antibody
    • MGC111559 antibody
    • Ras homolog enriched in brain 2 antibody
    • Ras homolog enriched in brain antibody
    • RHEB 2 antibody
    • Rheb antibody
    • RHEB_HUMAN antibody
    • RHEB2 antibody
    • RHEB2, formerly antibody
    see all

Anti-RHEB antibody images

  • Anti-RHEB antibody (ab76629) at 1 µg/ml + Recombinant tagged full length human RHEB at 0.2 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP at 1/5000 dilution

    Predicted band size : 20 kDa
    Observed band size : 46 kDa (why is the actual band size different from the predicted?)
  • Anti-RHEB antibody (ab76629) at 1 µg/ml + Jurkat cell lysate at 25 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

    Predicted band size : 20 kDa
    Observed band size : 17 kDa (why is the actual band size different from the predicted?)

References for Anti-RHEB antibody (ab76629)

ab76629 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"