Overview

  • Product nameAnti-RHEB antibody [EPR2971]
    See all RHEB primary antibodies
  • Description
    Rabbit monoclonal [EPR2971] to RHEB
  • Tested applicationsSuitable for: WB, IHC-P, ICC, Flow Cytmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to residues in Human RHEB

  • Positive control
    • Raji, SH-SY5Y cell lysates and Human brain tissue
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Alternative versions available:

    Anti-RHEB antibody (Biotin) [EPR2971] (ab199747)

    Anti-RHEB antibody (HRP) [EPR2971] (ab199230)

Properties

Applications

Our Abpromise guarantee covers the use of ab92313 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/5000. Predicted molecular weight: 20 kDa.Can be blocked with Human RHEB peptide (ab194764).
IHC-P 1/100 - 1/250.

Heat mediated antigen retrieval and use of an HRP/AP polymerized secondary antibody recommended.

ICC 1/100 - 1/250.
Flow Cyt 1/100 - 1/1000. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • Application notesIs unsuitable for IP.
  • Target

    • FunctionStimulates the phosphorylation of S6K1 and EIF4EBP1 through activation of mTORC1 signaling. Activates the protein kinase activity of mTORC1. Has low intrinsic GTPase activity.
    • Tissue specificityUbiquitous. Highest levels observed in skeletal and cardiac muscle.
    • Sequence similaritiesBelongs to the small GTPase superfamily. Rheb family.
    • Post-translational
      modifications
      Farnesylation is important for efficiently activating mTORC1-mediated signaling.
    • Cellular localizationCell membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • Ras homolog enriched in brain 2, formerly antibody
      • GTP binding protein Rheb antibody
      • GTP-binding protein Rheb antibody
      • MGC111559 antibody
      • Ras homolog enriched in brain 2 antibody
      • Ras homolog enriched in brain antibody
      • RHEB 2 antibody
      • Rheb antibody
      • RHEB_HUMAN antibody
      • RHEB2 antibody
      • RHEB2, formerly antibody
      see all

    Anti-RHEB antibody [EPR2971] images

    • Overlay histogram showing Raji cells stained with ab92313 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab92313, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in Raji cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
    • All lanes : Anti-RHEB antibody [EPR2971] (ab92313) at 1/2000 dilution

      Lane 1 : Raji cell lsyate
      Lane 2 : SH-SY5Y cell lsyate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 20 kDa
    • ab92313, at 1/100 dilution, staining RHEB in paraffin-embedded Human brain tissue, by Immunohistochemistry.

    References for Anti-RHEB antibody [EPR2971] (ab92313)

    This product has been referenced in:
    • Jiang L  et al. Rheb/mTORC1 Signaling Promotes Kidney Fibroblast Activation and Fibrosis. J Am Soc Nephrol 24:1114-26 (2013). IHC-P ; Human . Read more (PubMed: 23661807) »

    See 1 Publication for this product

    Product Wall

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"