Overview

  • Product name
    Anti-Rheumatoid Factor IgM ELISA Kit
  • Detection method
    Colorimetric
  • Sample type
    Serum, Plasma
  • Assay type
    Indirect
  • Sensitivity
    < 0.5 U/ml
  • Assay duration
    Multiple steps standard assay
  • Product overview

    Abcam’s anti-Rheumatoid Factor IgM ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative determination of autoantibodies to Rheumatoid Factor IgM in Human serum or plasma.

    The quantitative immunoenzymatic determination of IgM rheumatoid factors is based on the ELISA (Enzyme-linked Immunosorbent Assay) technique. Microtiter strips are precoated with Fc fragments of Human immunoglobulin G to bind corresponding antibodies of the specimen. After washing the wells to remove all unbound sample material horseradish peroxidase (HRP) labelled anti-Human IgM conjugate is added. This conjugate binds to the captured rheumatoid factors. The immune complex formed by the bound conjugate is visualized by adding Tetramethylbenzidine (TMB) substrate which gives a blue reaction product. The intensity of this product is proportional to the amount of IgM rheumatoid factors in the specimen. Sulphuric acid is added to stop the reaction. This produces a yellow endpoint colour. Absorbance at 450 nm is read using an ELISA microwell plate reader.

  • Tested applications
    Suitable for: Indirect ELISAmore details
  • Platform
    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab178653 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Indirect ELISA Use at an assay dependent concentration.

Images

Protocols

References

ab178653 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab178653.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up