Abcam’s anti-Rheumatoid Factor IgM ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative determination of autoantibodies to Rheumatoid Factor IgM in Human serum or plasma.
The quantitative immunoenzymatic determination of IgM rheumatoid factors is based on the ELISA (Enzyme-linked Immunosorbent Assay) technique. Microtiter strips are precoated with Fc fragments of Human immunoglobulin G to bind corresponding antibodies of the specimen. After washing the wells to remove all unbound sample material horseradish peroxidase (HRP) labelled anti-Human IgM conjugate is added. This conjugate binds to the captured rheumatoid factors. The immune complex formed by the bound conjugate is visualized by adding Tetramethylbenzidine (TMB) substrate which gives a blue reaction product. The intensity of this product is proportional to the amount of IgM rheumatoid factors in the specimen. Sulphuric acid is added to stop the reaction. This produces a yellow endpoint colour. Absorbance at 450 nm is read using an ELISA microwell plate reader.
|Components||Identifier||1 x 96 tests|
|20X Washing Solution||White cap||1 x 50ml|
|Cover Foil||1 unit|
|IgG Fc Fragment Coated Microplate (12 x 8 wells)||1 unit|
|IgG Sample Diluent||1 x 100ml|
|Rheumatoid Factor Anti-IgM HRP Conjugate||1 x 20ml|
|Rheumatoid Factor IgM Negative Control||1 x 2ml|
|Rheumatoid Factor IgM Positive Control||1 x 2ml|
|Rheumatoid Factor IgM Standard A - 0 U/mL||1 x 2ml|
|Rheumatoid Factor IgM Standard B - 3 U/mL||1 x 2ml|
|Rheumatoid Factor IgM Standard C - 10 U/mL||1 x 2ml|
|Rheumatoid Factor IgM Standard D - 30 U/mL||1 x 2ml|
|Rheumatoid Factor IgM Standard E - 100 U/mL||1 x 2ml|
|Rheumatoid Factor IgM Standard F - 300 U/mL||1 x 2ml|
|Stop Solution||1 x 15ml|
|Strip holder||1 unit|
|TMB Substrate Solution||Yellow cap||1 x 15ml|
Our Abpromise guarantee covers the use of ab178653 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Indirect ELISA||Use at an assay dependent concentration.|
ab178653 has not yet been referenced specifically in any publications.