Anti-ARHGAP29 antibody (ab85853)
Key features and details
- Rabbit polyclonal to ARHGAP29
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-ARHGAP29 antibody -
Description
Rabbit polyclonal to ARHGAP29 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide corresponding to Human ARHGAP29 aa 1211-1261.
Database link: NP_004806.3 -
Positive control
- HeLa, 293T and NIH3T3 whole cell lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab85853 was affinity purified using an epitope specific to ARHGAP29 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab85853 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/2000 - 1/10000. Predicted molecular weight: 142 kDa.
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IP |
Use at 2-5 µg/mg of lysate.
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Notes |
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WB
1/2000 - 1/10000. Predicted molecular weight: 142 kDa. |
IP
Use at 2-5 µg/mg of lysate. |
Target
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Function
GTPase activator for the Rho-type GTPases by converting them to an inactive GDP-bound state. Has strong activity toward RHOA, and weaker activity toward RAC1 and CDC42. May act as a specific effector of RAP2A to regulate Rho. In concert with RASIP1, suppresses RhoA signaling and dampens ROCK and MYH9 activities in endothelial cells and plays an essential role in blood vessel tubulogenesis. -
Tissue specificity
Widely expressed. Highly expressed in skeletal muscle and heart. Expressed at intermediate level in placenta, liver and pancreas. Weakly expressed in brain, lung and kidney. -
Sequence similarities
Contains 1 phorbol-ester/DAG-type zinc finger.
Contains 1 Rho-GAP domain. - Information by UniProt
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Database links
- Entrez Gene: 9411 Human
- Entrez Gene: 214137 Mouse
- Omim: 610496 Human
- SwissProt: Q52LW3 Human
- SwissProt: Q8CGF1 Mouse
- Unigene: 483238 Human
- Unigene: 229287 Mouse
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Alternative names
- ARHGAP 29 antibody
- Arhgap29 antibody
- ARHGAP29 protein antibody
see all
Images
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All lanes : Anti-ARHGAP29 antibody (ab85853) at 0.4 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : NIH3T3 whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 142 kDa
Observed band size: 170 kDa why is the actual band size different from the predicted?
Exposure time: 30 seconds -
Detection of ARHGAP29 by Western Blot of Immunprecipitate.
ab85853, at 1 µg/ml, staining ARHGAP29 in HeLa whole cell lysate immunoprecipitated using ab85853 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 10 seconds.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab85853 has been referenced in 2 publications.
- Shimizu K et al. ARHGAP29 expression may be a novel prognostic factor of cell proliferation and invasion in prostate cancer. Oncol Rep 44:2735-2745 (2020). PubMed: 33125156
- Hernández CJ et al. PINK1 Silencing Modifies Dendritic Spine Dynamics of Mouse Hippocampal Neurons. J Mol Neurosci 69:570-579 (2019). PubMed: 31486971