Anti-Rho GTPase activating protein 29 antibody (ab85853)

Overview

  • Product nameAnti-Rho GTPase activating protein 29 antibody
    See all Rho GTPase activating protein 29 primary antibodies
  • Description
    Rabbit polyclonal to Rho GTPase activating protein 29
  • Tested applicationsSuitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide, corresponding to a region between residues 1211 and 1261 of human Rho GTPase activating protein 29 (NP_004806.3).

  • Positive control
    • HeLa, 293T and NIH3T3 whole cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab85853 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IP
  • Application notesIP: Use at 2-5 µg/mg of lysate.
    WB: 1/2000 - 1/10000. Predicted molecular weight: 142 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionGTPase activator for the Rho-type GTPases by converting them to an inactive GDP-bound state. Has strong activity toward RHOA, and weaker activity toward RAC1 and CDC42. May act as a specific effector of RAP2A to regulate Rho. In concert with RASIP1, suppresses RhoA signaling and dampens ROCK and MYH9 activities in endothelial cells and plays an essential role in blood vessel tubulogenesis.
    • Tissue specificityWidely expressed. Highly expressed in skeletal muscle and heart. Expressed at intermediate level in placenta, liver and pancreas. Weakly expressed in brain, lung and kidney.
    • Sequence similaritiesContains 1 phorbol-ester/DAG-type zinc finger.
      Contains 1 Rho-GAP domain.
    • Information by UniProt
    • Database links
    • Alternative names
      • ARHGAP 29 antibody
      • Arhgap29 antibody
      • ARHGAP29 protein antibody
      • MGC166097 antibody
      • PARG 1 antibody
      • PARG1 antibody
      • PTPL1 associated RhoGAP 1 antibody
      • PTPL1-associated RhoGAP protein 1 antibody
      • RHG29_HUMAN antibody
      • Rho GTPase activating protein 29 antibody
      • Rho GTPase activating protein 29 isoform CRA_a antibody
      • Rho GTPase activating protein 29 isoform CRA_b antibody
      • Rho GTPase-activating protein 29 antibody
      • Rho-type GTPase-activating protein 29 antibody
      see all

    Anti-Rho GTPase activating protein 29 antibody images

    • All lanes : Anti-Rho GTPase activating protein 29 antibody (ab85853) at 0.4 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg
      Lane 4 : 293T whole cell lysate at 50 µg
      Lane 5 : NIH3T3 whole cell lysate at 50 µg

      Developed using the ECL technique

      Predicted band size : 142 kDa
      Observed band size : 170 kDa (why is the actual band size different from the predicted?)


      Exposure time : 30 seconds
    • Detection of Rho GTPase activating protein 29 by Western Blot of Immunprecipitate.
      ab85853, at 1 µg/ml, staining Rho GTPase activating protein 29 in HeLa whole cell lysate immunoprecipitated using ab85853 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
      Detection: Chemiluminescence with exposure time of 10 seconds.

    References for Anti-Rho GTPase activating protein 29 antibody (ab85853)

    ab85853 has not yet been referenced specifically in any publications.

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