Recombinant
RabMAb

Anti-Rho guanine exchange factor 16 antibody [EPR9950] (ab154785)

Overview

  • Product name
    Anti-Rho guanine exchange factor 16 antibody [EPR9950]
    See all Rho guanine exchange factor 16 primary antibodies
  • Description
    Rabbit monoclonal [EPR9950] to Rho guanine exchange factor 16
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IF, Flow Cytmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to residues in Human Rho guanine exchange factor 16 (UniProt Q5VV41).

  • Positive control
    • HeLa, MCF-7 and 293T cell lysates; Human cervix carcinoma tissue; Human breast tissue; HeLa cells.
  • General notes

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab154785 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 80 kDa.
IHC-P 1/50 - 1/100.
ICC/IF 1/100 - 1/250.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for IP.
  • Target

    • Function
      Guanyl-nucleotide exchange factor of the RHOG GTPase stimulating the exchange of RHOG-associated GDP for GTP. May play a role in chemotactic cell migration by mediating the activation of RAC1 by EPHA2. May also activate CDC42 and mediate activation of CDC42 by the viral protein HPV16 E6.
    • Sequence similarities
      Contains 1 DH (DBL-homology) domain.
      Contains 1 PH domain.
      Contains 1 SH3 domain.
    • Domain
      The PDZ-binding motif mediates interaction with TAX1BP3.
    • Cellular localization
      Cytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • ARHGEF 16 antibody
      • ARHGEF16 antibody
      • ARHGG_HUMAN antibody
      • Ephexin 4 antibody
      • Ephexin-4 antibody
      • EPHEXIN4 antibody
      • GEF16 antibody
      • NBR antibody
      • Putative Neuroblastoma Protein antibody
      • Rho guanine exchange factor (GEF) 16 antibody
      • Rho guanine nucleotide exchange factor (GEF) 16 antibody
      • Rho guanine nucleotide exchange factor 16 antibody
      see all

    Images

    • All lanes : Anti-Rho guanine exchange factor 16 antibody [EPR9950] (ab154785) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : MCF-7 cell lysate
      Lane 3 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 80 kDa

    • Immunohistochemical analysis of paraffin-embedded Human cervix carcinoma tissue labeling Rho guanine exchange factor 16 with ab154785 at 1/50 dilution.
    • Immunohistochemical analysis of paraffin-embedded Human normal breast tissue labeling Rho guanine exchange factor 16 with ab154785 at 1/50 dilution.
    • Immunofluorescent analysis of HeLa cells labeling Rho guanine exchange factor 16 with ab154785 at 1/100 dilution.
    • Flow cytometric analysis of permeabilized HeLa cells labeling Rho guanine exchange factor 16 with ab154785 at 1/10 dilution (red) compared to a rabbit IgG negative control (green).
    • Immunohistochemical analysis of paraffin embedded Human normal liver tissue using ab154785 showing +ve staining.

    • Immunohistochemical analysis of paraffin embedded Human skeletal muscle tissue using ab154785 showing +ve staining.

    • Immunohistochemical analysis of paraffin embedded Human thyroid gland carcinoma tissue using ab154785 showing +ve staining.

    • Immunohistochemical analysis of paraffin embedded Human normal uterus tissue using ab154785 showing +ve staining.

    References

    ab154785 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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