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Synthetic peptide phosphorylated at Ser188, corresponding to amino acid residues around serine 188 of human RhoA, conjugated to KLH.
Post-translational regulation of Rho activity has been shown specifically for RhoA. This Rho protein is phosphorylated in vitro on serine 188 by cAMP- and cGMP-dependent kinases (PKA and PKG). Ser188 phosphorylation enhances RhoGDI binding and inhibits RhoA-mediated stress fiber formation. Thus, Ser188 is an important site for negative regulation of RhoA activity.
Our Abpromise guarantee covers the use of ab41435 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Predicted molecular weight: 22 kDa.|
A fusion protein was used in this western blot; hence the difference in molecular weight compared to predicted molecular weight.
Blocking: 5% milk for 1 hour at 23°C
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