Overview

  • Product name
    Anti-RHOB antibody [2916C2a]
    See all RHOB primary antibodies
  • Description
    Mouse monoclonal [2916C2a] to RHOB
  • Host species
    Mouse
  • Tested applications
    Suitable for: Dot blot, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment (Human)

Properties

Applications

Our Abpromise guarantee covers the use of ab53743 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Dot blot Use at an assay dependent dilution.
WB Use at an assay dependent dilution. Predicted molecular weight: 22 kDa.

Target

  • Function
    Mediates apoptosis in neoplastically transformed cells after DNA damage. Not essential for development but affects cell adhesion and growth factor signaling in transformed cells. Plays a negative role in tumorigenesis as deletion causes tumor formation. Involved in intracellular protein trafficking of a number of proteins. Targets PKN1 to endosomes and is involved in trafficking of the EGF receptor from late endosomes to lysosomes. Also required for stability and nuclear trafficking of AKT1/AKT which promotes endothelial cell survival during vascular development.
  • Sequence similarities
    Belongs to the small GTPase superfamily. Rho family.
  • Post-translational
    modifications
    Prenylation specifies the subcellular location of RHOB. The farnesylated form is localized to the plasma membrane while the geranylgeranylated form is localized to the endosome.
  • Cellular localization
    Late endosome membrane. Cell membrane. Nucleus. Late endosomal membrane (geranylgeranylated form). Plasma membrane (farnesylated form). Also detected at the nuclear margin and in the nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Aplysia RAS-related homolog 6 antibody
    • ARH6 antibody
    • ARHB antibody
    • H6 antibody
    • MST081 antibody
    • MSTP081 antibody
    • oncogene RHO H6 antibody
    • ras homolog family member B antibody
    • ras homolog gene family, member B antibody
    • Rho cDNA clone 6 antibody
    • Rho related GTP binding protein RhoB Precursor antibody
    • Rho-related GTP-binding protein RhoB antibody
    • Rhob antibody
    • RHOB_HUMAN antibody
    • RHOH6 antibody
    see all

Images

  • Anti-RHOB antibody [2916C2a] (ab53743) + immunized recombinant protein

    Predicted band size: 22 kDa
    Observed band size: 39 kDa (why is the actual band size different from the predicted?)



    The band on the Wetstern blot does not correspond to the predicted size, as the recombinant protein was used as the test antigen

References

This product has been referenced in:
  • Quinn K  et al. Rho GTPases modulate entry of Ebola virus and vesicular stomatitis virus pseudotyped vectors. J Virol 83:10176-86 (2009). Read more (PubMed: 19625394) »
  • Murray D  et al. NET1-mediated RhoA activation facilitates lysophosphatidic acid-induced cell migration and invasion in gastric cancer. Br J Cancer 99:1322-9 (2008). WB ; Human . Read more (PubMed: 18827818) »

See all 2 Publications for this product

Customer reviews and Q&As

Application
ELISA
Sample
Human Cell (HeLa cells)
Specification
HeLa cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Type
Sandwich (Detection)
Username

Abcam user community

Verified customer

Submitted Apr 04 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up