Overview

  • Product nameAnti-RHOB antibody [2916C2a]
    See all RHOB primary antibodies
  • Description
    Mouse monoclonal [2916C2a] to RHOB
  • Tested applicationsSuitable for: Dot blot, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment (Human)

Properties

Applications

Our Abpromise guarantee covers the use of ab53743 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Dot blot Use at an assay dependent dilution.
WB Use at an assay dependent dilution. Predicted molecular weight: 22 kDa.

Target

  • FunctionMediates apoptosis in neoplastically transformed cells after DNA damage. Not essential for development but affects cell adhesion and growth factor signaling in transformed cells. Plays a negative role in tumorigenesis as deletion causes tumor formation. Involved in intracellular protein trafficking of a number of proteins. Targets PKN1 to endosomes and is involved in trafficking of the EGF receptor from late endosomes to lysosomes. Also required for stability and nuclear trafficking of AKT1/AKT which promotes endothelial cell survival during vascular development.
  • Sequence similaritiesBelongs to the small GTPase superfamily. Rho family.
  • Post-translational
    modifications
    Prenylation specifies the subcellular location of RHOB. The farnesylated form is localized to the plasma membrane while the geranylgeranylated form is localized to the endosome.
  • Cellular localizationLate endosome membrane. Cell membrane. Nucleus. Late endosomal membrane (geranylgeranylated form). Plasma membrane (farnesylated form). Also detected at the nuclear margin and in the nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Aplysia RAS-related homolog 6 antibody
    • ARH6 antibody
    • ARHB antibody
    • H6 antibody
    • MST081 antibody
    • MSTP081 antibody
    • oncogene RHO H6 antibody
    • ras homolog family member B antibody
    • ras homolog gene family, member B antibody
    • Rho cDNA clone 6 antibody
    • Rho related GTP binding protein RhoB Precursor antibody
    • Rho-related GTP-binding protein RhoB antibody
    • Rhob antibody
    • RHOB_HUMAN antibody
    • RHOH6 antibody
    see all

Anti-RHOB antibody [2916C2a] images

References for Anti-RHOB antibody [2916C2a] (ab53743)

This product has been referenced in:
  • Quinn K  et al. Rho GTPases modulate entry of Ebola virus and vesicular stomatitis virus pseudotyped vectors. J Virol 83:10176-86 (2009). Read more (PubMed: 19625394) »
  • Murray D  et al. NET1-mediated RhoA activation facilitates lysophosphatidic acid-induced cell migration and invasion in gastric cancer. Br J Cancer 99:1322-9 (2008). WB ; Human . Read more (PubMed: 18827818) »

See all 2 Publications for this product

Product Wall

Application ELISA
Sample Human Cell (HeLa cells)
Specification HeLa cells
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Type Sandwich (Detection)
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Submitted Apr 04 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"