Overview

  • Product nameAnti-RHOC [2E12] antibodySee all RHOC primary antibodies ...
  • Description
    Mouse monoclonal [2E12] to RHOC
  • Tested applicationsFlow Cyt, WB more details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Recombinant full length protein, corresponding to amino acids 1-194 of Human RHOC

Properties

Applications

Our Abpromise guarantee covers the use of ab54837 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
Flow Cyt Use 1µg for 106 cells.
WB Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 22 kDa.

Target

  • FunctionRegulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers.
  • Sequence similaritiesBelongs to the small GTPase superfamily. Rho family.
  • Cellular localizationCell membrane.
  • Target information above from: UniProt accession P08134 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • ARH 9 antibody
    • ARH9 antibody
    • ARHC antibody
    • H9 antibody
    • MGC1448 antibody
    • MGC61427 antibody
    • Oncogene RHO H9 antibody
    • Ras homolog gene family member C antibody
    • RAS related homolog 9 antibody
    • RHO C antibody
    • Rho cDNA clone 9 antibody
    • Rho related GTP binding protein RhoC antibody
    • Rho-related GTP-binding protein RhoC antibody
    • rhoC antibody
    • RhoC GTPase antibody
    • RHOC_HUMAN antibody
    • RHOH 9 antibody
    • RHOH9 antibody
    • Small GTP binding protein RhoC antibody
    see all

Anti-RHOC [2E12] antibody images

  • RHOC antibody (ab54837) at 1ug/lane + Jurkat cell lysate at 25ug/lane.
  • Overlay histogram showing HCT116 cells stained with ab54837 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab54837, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HCT116 cells fixed with 80% methanol/permeabilized in 0.1% PBS-Tween used under the same conditions.

References for Anti-RHOC [2E12] antibody (ab54837)

This product has been referenced in:
  • Tripathi V  et al. DLC1 induces expression of E-cadherin in prostate cancer cells through Rho pathway and suppresses invasion. Oncogene N/A:N/A (2013). Read more (PubMed: 23376848) »
  • Del Galdo S  et al. The activation of RhoC in vascular endothelial cells is required for the S1P receptor type 2-induced inhibition of angiogenesis. Cell Signal 25:2478-84 (2013). Read more (PubMed: 23993968) »

See all 5 Publications for this product

Product Wall

Application Western blot
Sample Rat Cell lysate - whole cell (C6 Rat glioma cell line)
Loading amount 40 µg
Specification C6 Rat glioma cell line
Gel Running Conditions Reduced Denaturing (15)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Mar 03 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"