RHOC antibody (ab54837) at 1ug/lane + Jurkat cell lysate at 25ug/lane.
Flow Cytometry-RHOC antibody(ab54837)
Overlay histogram showing HCT116 cells stained with ab54837 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab54837, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HCT116 cells fixed with 80% methanol/permeabilized in 0.1% PBS-Tween used under the same conditions.
References for Anti-RHOC antibody [2E12] (ab54837)
This product has been referenced in:
Chen M et al. Use of synthetic isoprenoids to target protein prenylation and Rho GTPases in breast cancer invasion. PLoS One9:e89892 (2014).
Read more (PubMed: 24587105) »
Passadouro M et al. MicroRNA modulation combined with sunitinib as a novel therapeutic strategy for pancreatic cancer. Int J Nanomedicine9:3203-17 (2014).
Read more (PubMed: 25061297) »