• Product name
    Anti-RHOC antibody [2E12]
    See all RHOC primary antibodies
  • Description
    Mouse monoclonal [2E12] to RHOC
  • Tested applications
    Suitable for: Flow Cyt, WBmore details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Recombinant full length protein, corresponding to amino acids 1-194 of Human RHOC



Our Abpromise guarantee covers the use of ab54837 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 1µg for 106 cells. ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
WB Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 22 kDa.


  • Function
    Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers.
  • Sequence similarities
    Belongs to the small GTPase superfamily. Rho family.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Aplysia RAS-related homolog 9 antibody
    • ARH 9 antibody
    • ARH9 antibody
    • ARHC antibody
    • H9 antibody
    • MGC1448 antibody
    • MGC61427 antibody
    • Oncogene RHO H9 antibody
    • Ras homolog gene family member C antibody
    • RAS related homolog 9 antibody
    • RHO C antibody
    • Rho cDNA clone 9 antibody
    • Rho related GTP binding protein RhoC antibody
    • Rho-related GTP-binding protein RhoC antibody
    • rhoC antibody
    • RhoC GTPase antibody
    • RHOC_HUMAN antibody
    • RHOH 9 antibody
    • RHOH9 antibody
    • Small GTP binding protein RhoC antibody
    see all

Anti-RHOC antibody [2E12] images

  • RHOC antibody (ab54837) at 1ug/lane + Jurkat cell lysate at 25ug/lane.
  • Overlay histogram showing HCT116 cells stained with ab54837 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab54837, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HCT116 cells fixed with 80% methanol/permeabilized in 0.1% PBS-Tween used under the same conditions.

References for Anti-RHOC antibody [2E12] (ab54837)

This product has been referenced in:
  • Xu XD  et al. Anti-RhoC siRNAs inhibit the proliferation and invasiveness of breast cancer cells via modulating the KAI1, MMP9, and CXCR4 expression. Onco Targets Ther 10:1827-1834 (2017). WB ; Human . Read more (PubMed: 28367066) »
  • Chen M  et al. Use of synthetic isoprenoids to target protein prenylation and Rho GTPases in breast cancer invasion. PLoS One 9:e89892 (2014). Human . Read more (PubMed: 24587105) »

See all 8 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Western blot
Rat Cell lysate - whole cell (C6 Rat glioma cell line)
Loading amount
40 µg
C6 Rat glioma cell line
Gel Running Conditions
Reduced Denaturing (15)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

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Verified customer

Submitted Mar 03 2011


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