Overview

  • Product name
    Anti-RHOC antibody [2E12]
    See all RHOC primary antibodies
  • Description
    Mouse monoclonal [2E12] to RHOC
  • Tested applications
    Suitable for: Flow Cyt, WBmore details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Recombinant full length protein, corresponding to amino acids 1-194 of Human RHOC

  • General notes

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by July 2018 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. 

Properties

Applications

Our Abpromise guarantee covers the use of ab54837 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 1µg for 106 cells. ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
WB Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 22 kDa.

Target

  • Function
    Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers.
  • Sequence similarities
    Belongs to the small GTPase superfamily. Rho family.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Aplysia RAS-related homolog 9 antibody
    • ARH 9 antibody
    • ARH9 antibody
    • ARHC antibody
    • H9 antibody
    • MGC1448 antibody
    • MGC61427 antibody
    • Oncogene RHO H9 antibody
    • Ras homolog gene family member C antibody
    • RAS related homolog 9 antibody
    • RHO C antibody
    • Rho cDNA clone 9 antibody
    • Rho related GTP binding protein RhoC antibody
    • Rho-related GTP-binding protein RhoC antibody
    • rhoC antibody
    • RhoC GTPase antibody
    • RHOC_HUMAN antibody
    • RHOH 9 antibody
    • RHOH9 antibody
    • Small GTP binding protein RhoC antibody
    see all

Images

  • RHOC antibody (ab54837) at 1ug/lane + Jurkat cell lysate at 25ug/lane.
  • Overlay histogram showing HCT116 cells stained with ab54837 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab54837, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HCT116 cells fixed with 80% methanol/permeabilized in 0.1% PBS-Tween used under the same conditions.

References

This product has been referenced in:
  • Xu XD  et al. Anti-RhoC siRNAs inhibit the proliferation and invasiveness of breast cancer cells via modulating the KAI1, MMP9, and CXCR4 expression. Onco Targets Ther 10:1827-1834 (2017). WB ; Human . Read more (PubMed: 28367066) »
  • Chen M  et al. Use of synthetic isoprenoids to target protein prenylation and Rho GTPases in breast cancer invasion. PLoS One 9:e89892 (2014). Human . Read more (PubMed: 24587105) »

See all 8 Publications for this product

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Cell lysate - whole cell (C6 Rat glioma cell line)
Loading amount
40 µg
Specification
C6 Rat glioma cell line
Gel Running Conditions
Reduced Denaturing (15)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Mar 03 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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