Overview

  • Product name
    Anti-Rhodamine antibody [11H10]
  • Description
    Mouse monoclonal [11H10] to Rhodamine
  • Specificity
    This antibody reacts specifically with Rhodamine and its derivatives. Ab9093 was raised against TRITC and reacts with rhodamine isomers 5 and 6, TAMRA, and TRITC, but it does not react with Texas Red
  • Tested applications
    Suitable for: IHC-P, Dot blot, ELISAmore details
  • Immunogen

    This protein A purified monoclonal antibody was produced after repeated immunizations of balb/c mice with rhodamine conjugated KLH.

  • General notes
    The molecular formula of 5-carboxytetramethylrhodamine, succinimidyl ester (5-TAMRA, SE)is C29H25N3O7 The molecular weight of 5-TAMRA, SE is 527.53 This product contains 0.01% (w/v) Sodium Azide preservative.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    11H10
  • Myeloma
    Sp2/0
  • Isotype
    IgG1
  • Light chain type
    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab9093 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. PubMed: 20650017
Dot blot 1/2000.
ELISA 1/500 - 1/1000.

Anti-Rhodamine antibody [11H10] images

  • A dilution series of two TAMRA labeled peptides were bound to a PVDF membrane using a dot blot apparatus. Peptide #1 is conjugated directly to TAMRA via an amide bond whereas peptide #2 contains an aminohexanoyl linker between the TAMRA and the peptide. Also note that the peptides differ in sequence and in length, peptide #1 has 15 amino acids and peptide #2 has 29 amino acids. A dilution series for each peptide was made starting with approximately 0.5 nmoles of peptide and followed by a series of 1:2 dilutions. After binding of the peptides to the PVDF membrane, the membrane was then blocked in 5% milk in 1X TBST for 1 hour at room temperature. The membrane was then incubated in with the ab9093 antibody at a 1:2000 dilution in 5% milk in 1X TBST for 1 hour at room temperature. Following incubation with the primary antibody the blot was then washed 3 times in 1X TBST and then incubated with a 1:10,000 dilution of an anti-mouse-HRP secondary antibody for 45 minutes at room temperature

References for Anti-Rhodamine antibody [11H10] (ab9093)

This product has been referenced in:
  • Opsahl ML & Kennedy PG Early and late HHV-6 gene transcripts in multiple sclerosis lesions and normal appearing white matter. Brain 128:516-27 (2005). Read more (PubMed: 15659422) »

See 1 Publication for this product

Product Wall

ab9093 does not recognize texas red.

Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (293T)
Total protein in input
1000 µg
Specification
293T
Treatment
Transfected Flag-tag TNFa protein with Fugene
Immuno-precipitation step
Protein A/G
Username

Abcam user community

Verified customer

Submitted May 07 2013

Thank you for submitting your image and experimental details. I have no published your review on our datasheet and awarded you 220 Abpoints. The following testing discount code is active and can be used to receive a free primary antibody on a future or...

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Thank you for your interest in testing ab9093 in dot blot.

Submitting your protocol and data using our Abreview system will take about 10 minutes. To find out more about our Abreview system, please visit:
http://www.abcam.com/abreviews....

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I am very pleased to hear you would like to accept our offer and test ab9093 inICC/IF. This code will give you 1 freeprimary antibodybefore the expiration date. To redeem this offer, please submit an Abreview forICC/IF and include this code in the...

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Thanks for your email.

I would just like to confirm if you will be using this antibody to stain cells or tissue sections.

Thanks for the additional information!

Thank you for your inquiry.


I heard back from the lab that unfortunately they do not have any information regarding rhodamine quenching by ab9093.


Since this is not something we typically test, I am unsure if any of our ...

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Thank you for your reply. Yes, this antibody will be covered under our Abpromise guarantee for detection of lissamine rhodamine in IHC-P. If you have any problems using it, please contact us within six months and we will be happy to replace or ...

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Thank you for your reply. We only have full size vials of all our antibodies, so we do not have small samples to send out for tests. However, since this antibody is supposed to recognize rhodamine derivatives, we will guarantee it to work agains...

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Thank you for your call yesterday and for your patience while I have looked into your enquiry. Ab9093 was raised against TRITC and reacts with rhodamine isomers 5 and 6, TAMRA, and TRITC, but it does not react with Texas Red. Unfortunately it h...

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1-10 of 12 Abreviews or Q&A

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