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The Abcam Rhodamine Fast Conjugation Kit (ab188286) provides an easy-to-use, one step procedure that allows researchers to covalently label proteins, peptides or other biomolecules with rhodamine with only 30 seconds hands on time; furthermore conjugates are ready to use in less than twenty minutes.
Rhodamine dyes are used extensively in biotechnology applications such as fluorescence microscopy, flow cytometry, fluorescence correlation spectroscopy and ELISA.
The rhodamine used in the Abcam Rhodamine Fast Conjugation Kit (ab188286) has an absorption peak at 520 and 544 nm. Maximum emission is obtained when the Rhodamine is excited at 544 nm.
The kit comes in 3 sizes for labelling recommended amounts of antibody:
300 μg containing 3 reactions (each for 100 - 200 μg)
1 mg containing 1 reaction (for 1 - 2 mg)
30 μg containing 3 reactions (each for 10 - 20 μg)
The antibody to be labelled should be purified, in an appropriate buffer for conjugation and at a suitable concentration, as described in the protocol booklet. If not, consider using our antibody purification and concentration kits.
|Components||300 µg||1 mg||30 µg|
|Rhodamine Conjugate||3 vials||1 vial||3 vials|
|Rhodamine Modifier reagent||1 vial||1 vial||1 vial|
|Rhodamine Quencher reagent||1 vial||1 vial||1 vial|
Our Abpromise guarantee covers the use of ab188286 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Conjugation||Use at an assay dependent concentration.|
ab188286 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"