Overview

  • Product nameAnti-Rhodopsin antibody [1D4]
    See all Rhodopsin primary antibodies
  • Description
    Mouse monoclonal [1D4] to Rhodopsin
  • Specificityab5417 detects Rhodopsin from Human and Bovine retinal samples. Data from Yin J et al., 2012 (PMID 22743318) indicates that in Zebrafish ab5417 appears to recognize Red Opsin rather than Rhodopsin.
  • Tested applicationsSuitable for: IHC-FoFr, IHC-Fr, ELISA, ICC, IP, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Cow, Human, Zebrafish, Amphibians
  • Immunogen

    Other Immunogen Type corresponding to Cow Rhodopsin.

  • EpitopeThe epitope for this antibody has been localized to the C-terminal nine amino acids of bovine rhodopsin known as the 1D4 epitope.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.05% Sodium azide
    Constituents: 99% PBS, 0.1% BSA
  • Concentration information loading...
  • PurityProtein G purified
  • Primary antibody notesVision involves the conversion of light into electrochemical signals that are processed by the retina and subsequently sent to and interpreted by the brain. The process of converting light to an electrochemical signal begins when the membrane-bound protein, rhodopsin, absorbs light within the retina. Photoexcitation of rhodopsin causes the cytoplasmic surface of the protein to become catalytically active. In the active state, rhodopsin activates transducin, a GTP binding protein. Once activated, transducin promotes the hydrolysis of cGMP by phosphodiesterase (PDE). The decrease of intracellular cGMP concentrations causes the ion channels within the outer segment of the rod or cone to close, thus causing membrane hyperpolarization and, eventually, signal transmission. Rhodopsin’s activity is believed to be shut off by its phosphorylation followed by binding of the soluble protein arrestin.
  • ClonalityMonoclonal
  • Clone number1D4
  • IsotypeIgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab5417 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FoFr 1/1000. PubMed: 19587120
IHC-Fr Use at an assay dependent concentration. PubMed: 22743318
ELISA Use at an assay dependent concentration.
ICC 1/1000.
IP Use at an assay dependent concentration.

Use at an assay dependent dilution.

WB 1/1000. Detects a band of approximately 40 kDa.
IHC-P 1/100 - 1/1000.

Target

  • FunctionPhotoreceptor required for image-forming vision at low light intensity. Required for photoreceptor cell viability after birth. Light-induced isomerization of 11-cis to all-trans retinal triggers a conformational change leading to G-protein activation and release of all-trans retinal.
  • Tissue specificityRod shaped photoreceptor cells which mediates vision in dim light.
  • Involvement in diseaseRetinitis pigmentosa 4
    Night blindness, congenital stationary, autosomal dominant 1
  • Sequence similaritiesBelongs to the G-protein coupled receptor 1 family. Opsin subfamily.
  • Post-translational
    modifications
    Phosphorylated on some or all of the serine and threonine residues present in the C-terminal region.
    Contains one covalently linked retinal chromophore.
  • Cellular localizationMembrane. Synthesized in the inner segment (IS) of rod photoreceptor cells before vectorial transport to the rod outer segment (OS) photosensory cilia.
  • Information by UniProt
  • Database links
  • Alternative names
    • CSNBAD1 antibody
    • MGC138309 antibody
    • MGC138311 antibody
    • OPN 2 antibody
    • OPN2 antibody
    • opsd antibody
    • OPSD_HUMAN antibody
    • opsin 2 antibody
    • Opsin 2 rod pigment antibody
    • Opsin-2 antibody
    • Opsin2 antibody
    • Retinitis Pigmentosa 4 antibody
    • Retinitis pigmentosa 4 autosomal dominant antibody
    • RHO antibody
    • Rhodopsin antibody
    • RP 4 antibody
    • RP4 antibody
    see all

Anti-Rhodopsin antibody [1D4] images

  • Immunohistochemical analysis of formalin-fixed mouse retinal tissue, labelling rhodopsin with ab5417 at a 1:50 dilution in 3% BSA-PBS solution and incubated at 4°C overnight in a high humidity environment. A DyLight® 488 secondary antibody was used (green) incubated at room temperature in the dark. The tissue was counterstained with DAPI against DNA, showing nuclear compartments. Prior to staining the formalin-fixed tissue was permeabilized with 0.1% Triton X-100 in TBS for between 5 and 10 minutes, then blocked with 3% BSA-PBS for 30 minutes at room temperature. The left image is a negative control with only the secondary antibody and the right image is in the presence of ab5417 and the secondary.

  • Immunohistochemical analysis of paraffin-embedded mouse retinal tissue labelling Rhodopsin with ab5417. Secondary used was HRP conjugated. Prior preparation was initiated by antigen retrival using 10mM sodium citrate at pH 6.0, then the sample was microwaved for 8 to 15 minutes. Subsequent to retrival the retinal tissue was blocked for 15 minutes at room temperature with 3% hydrogen peroxide. The sample was then incubated with ab5417 in 3% BSA-PBS at 4°C at a dilution of 1:1000, overnight. Hematoxylin was used to counterstain the tissue. The left side of the image is shown as a negative control and is the tissue in the absence of ab5417, the right side is in the prescence of the counterstain, ab5417 and the HRP conjugated secondary.

  • Immunohistochemistry (Frozen sections) image of anti-Rhodopsin antibody (ab5417) staining on a Zebrafish eye. This tissue section was stained with anti-Rhodopsin antibody (green) and Red Opsin antibody (red) for 16 hours at 4°C. Nuclei were stained with DAPI (blue). This image suggests that ab5417 detects Red Opsin and not Rhodopsin in the Zebrafish. For more information please see Yin J. et al., 2012.

    See Abreview

  • Immunohistochemical analysis of formalin-fixed human retinal tissue, labelling rhodopsin with ab5417 at a 1:50 dilution in 3% BSA-PBS solution and incubated at 4°C overnight in a high humidity environment. A DyLight® 488 secondary antibody was used (green) incubated at room temperature in the dark. The tissue was counterstained with DAPI against DNA, showing nuclear compartments. Prior to staining the formalin-fixed tissue was permeabilized with 0.1% Triton X-100 in TBS for between 5 and 10 minutes, then blocked with 3% BSA-PBS for 30 minutes at room temperature. The left image is a negative control with only the secondary antibody and the right image is in the presence of ab5417 and the secondary.

  • Anti-Rhodopsin antibody [1D4] (ab5417) at 1/500 dilution + HL60 cell lysate at 25 µg

    Observed band size : 40 kDa (why is the actual band size different from the predicted?)

References for Anti-Rhodopsin antibody [1D4] (ab5417)

This product has been referenced in:
  • Trapani I  et al. Effective delivery of large genes to the retina by dual AAV vectors. EMBO Mol Med 6:194-211 (2014). IHC-Fr ; Mouse . Read more (PubMed: 24150896) »
  • Wavre-Shapton ST  et al. Phagosome maturation during endosome interaction revealed by partial rhodopsin processing in retinal pigment epithelium. J Cell Sci 127:3852-61 (2014). Mouse . Read more (PubMed: 25074813) »

See all 10 Publications for this product

Product Wall

Application Western blot
Loading amount 10 µg
Gel Running Conditions Non-reduced Denaturing (12%)
Sample Human Cell lysate - whole cell (neuroblast)
Specification neuroblast
Blocking step BSA as blocking agent for 50 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jun 17 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Sample Zebrafish Tissue sections (Long double cone (red cone) photoreceptor cells)
Specification Long double cone (red cone) photoreceptor cells
Permeabilization Yes - PBST
Fixative Paraformaldehyde
Username

Dr. Jun Yin

Verified customer

Submitted Jul 02 2013

Thank you very much for your call today. It was a pleasure speaking to you. I've created the discount code below, and if you have any further questions about redeeming the code or submitting the Abreview, please let me know and I'll be happy to help. <...

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