Overview

  • Product name
  • Description
    Rabbit polyclonal to Rhodopsin
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Mouse, Rabbit, Pig
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Human Rhodopsin.

  • Positive control
    • WB: Y79 and WERI whole cell lysates. IHC-P: Rat normal eye tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
    Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab112576 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 0.5 µg/ml.

The optimal concentration will need to be determined. 0.5ug/mL gave a good result with a polymer-HRP secondary antibody reagent.

WB Use a concentration of 1 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 39 kDa).

Target

  • Function
    Photoreceptor required for image-forming vision at low light intensity. Required for photoreceptor cell viability after birth. Light-induced isomerization of 11-cis to all-trans retinal triggers a conformational change leading to G-protein activation and release of all-trans retinal.
  • Tissue specificity
    Rod shaped photoreceptor cells which mediates vision in dim light.
  • Involvement in disease
    Retinitis pigmentosa 4
    Night blindness, congenital stationary, autosomal dominant 1
  • Sequence similarities
    Belongs to the G-protein coupled receptor 1 family. Opsin subfamily.
  • Post-translational
    modifications
    Phosphorylated on some or all of the serine and threonine residues present in the C-terminal region.
    Contains one covalently linked retinal chromophore.
  • Cellular localization
    Membrane. Synthesized in the inner segment (IS) of rod photoreceptor cells before vectorial transport to the rod outer segment (OS) photosensory cilia.
  • Information by UniProt
  • Database links
  • Alternative names
    • CSNBAD1 antibody
    • MGC138309 antibody
    • MGC138311 antibody
    • OPN 2 antibody
    • OPN2 antibody
    • opsd antibody
    • OPSD_HUMAN antibody
    • opsin 2 antibody
    • Opsin 2 rod pigment antibody
    • Opsin-2 antibody
    • Opsin2 antibody
    • Retinitis Pigmentosa 4 antibody
    • Retinitis pigmentosa 4 autosomal dominant antibody
    • RHO antibody
    • Rhodopsin antibody
    • RP 4 antibody
    • RP4 antibody
    see all

Images

  • IHC image of Rhodopsin staining in Rat normal eye formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab112576, 0.5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • All lanes : Anti-Rhodopsin antibody (ab112576) at 1 µg/ml

    Lane 1 : Y79 (Human retinoblastoma cell line) Whole Cell Lysate
    Lane 2 : WERI (Human Retinoblastoma) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 39 kDa
    Observed band size : 48 kDa (why is the actual band size different from the predicted?)


    Exposure time : 2 minutesThe predicted molecular weight of Rhodopsin is 39 kDa (SwissProt), however we expect to observe a banding pattern around 48 kDa. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.

References

This product has been referenced in:
  • Eleftheriou CG  et al. Meclofenamic acid improves the signal to noise ratio for visual responses produced by ectopic expression of human rod opsin. Mol Vis 23:334-345 (2017). Histology ; Mouse . Read more (PubMed: 28659709) »

See 1 Publication for this product

Customer reviews and Q&As

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Filter by Species

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Application
Immunohistochemistry (Frozen sections)
Sample
Xenopus laevis Tissue sections (eye)
Permeabilization
Yes - 1% Triton X
Specification
eye
Blocking step
Serum as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5.0% · Temperature: RT°C
Fixative
Paraformaldehyde
Username

Mr. Donald Dugger

Verified customer

Submitted Oct 21 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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