Overview

  • Product name
  • Description
    Rabbit polyclonal to Rhodopsin
  • Host species
    Rabbit
  • Specificity
    Detects Rhodopsin from bovine retina samples. Data from Yin J et al., 2012 (PMID 22743318) indicates that in Zebrafish ab3424 appears to recognize Red Opsin rather than Rhodopsin.
  • Tested applications
    Suitable for: WB, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Cow
    Predicted to work with: Rat, Rabbit, Cat, Dog, Human, Cynomolgus monkey, Chinese hamster, African bush elephantDoes not react with: Sheep
  • Immunogen

    Synthetic peptide corresponding to Cow Rhodopsin aa 338-348.
    Sequence:

    SKTETSQVAPA


    (Peptide available as ab4967)

  • Positive control
    • Bovine outer rod segment extracts.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Constituents: 0.1% BSA, 99% PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Primary antibody notes
    Vision involves the conversion of light into electrochemical signals that are processed by the retina and subsequently sent to and interpreted by the brain. The process of converting light to an electrochemical signal begins when the membrane-bound protein rhodopsin absorbs light within the retina. Photoexcitation of rhodopsin causes the cytoplasmic surface of the protein to become catalytically active. In the active state, rhodopsin activates transducin, a GTP binding protein. Once activated, transducin promotes the hydrolysis of cGMP by phosphodiesterase (PDE). The decrease of intracellular cGMP concentrations causes the ion channels within the outer segment of the rod or cone to close, thus causing membrane hyperpolarization and, eventually, signal transmission. Rhodopsin activity is believed to be shut off by phosphorylation followed by binding of the soluble proteins arrestin.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab3424 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 38 kDa).Can be blocked with Rhodopsin peptide (ab4967).

By Western blot, this antibody detects an ~36 kDa protein representing rhodopsin from bovine outer rod segment extracts.

IHC-Fr Use at an assay dependent concentration. PubMed: 22743318

Target

Images

  • Immunohistochemistry (Frozen sections) image of anti-Rhodopsin antibody (ab3424) staining on a Zebrafish eye. This tissue section was stained with anti-Rhodopsin antibody ab3424 (green) and anti-Rhodopsin monoclonal antibody ab5417 (red) for 16 hours at 4°C. Nuclei were stained with DAPI (blue). This image suggests that ab3424 (like ab5417) may detect Red Opsin and not Rhodopsin in the Zebrafish. For more information please see Yin J. et al., 2012.

    See Abreview

References

This product has been referenced in:
  • Zhang YQ  et al. Down-regulation of protein kinase C alpha/ezrin signals in light-induced phagocytic crisis of retinal pigment epithelium cells. Int J Ophthalmol 10:1040-1045 (2017). Read more (PubMed: 28730104) »
  • MacDonald RB  et al. Müller glia provide essential tensile strength to the developing retina. J Cell Biol 210:1075-83 (2015). Read more (PubMed: 26416961) »

See all 3 Publications for this product

Customer reviews and Q&As

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Rat Tissue sections (Brain)
Antigen retrieval step
None
Permeabilization
Yes - 0.4% triton
Specification
Brain
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Fixative
Paraformaldehyde
Username

Brian Maunze

Verified customer

Submitted Jul 20 2015

Application
Immunohistochemistry (Frozen sections)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Sample
Zebrafish Tissue sections (Long double cone photoreceptor cells in zebrafish)
Specification
Long double cone photoreceptor cells in zebrafish
Permeabilization
Yes - PBST
Fixative
Paraformaldehyde
Username

Dr. Jun Yin

Verified customer

Submitted Jul 04 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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