• Product nameAnti-Rhodopsin antibody
    See all Rhodopsin primary antibodies
  • Description
    Rabbit polyclonal to Rhodopsin
  • SpecificityDetects Rhodopsin from bovine retina samples. Data from Yin J et al., 2012 (PMID 22743318) indicates that in Zebrafish ab3424 appears to recognize Red Opsin rather than Rhodopsin.
  • Tested applicationsSuitable for: WB, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Cow
    Predicted to work with: Rat, Rabbit, Cat, Dog, Human, Cynomolgus Monkey, Chinese Hamster, Loxodonta Africana (African bush elephant)Does not react with: Sheep
  • Immunogen

    Synthetic peptide corresponding to Cow Rhodopsin aa 338-348.


    (Peptide available as ab4967)

  • Positive control
    • Bovine outer rod segment extracts.


  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferConstituents: 0.1% BSA, 99% PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Primary antibody notesVision involves the conversion of light into electrochemical signals that are processed by the retina and subsequently sent to and interpreted by the brain. The process of converting light to an electrochemical signal begins when the membrane-bound protein rhodopsin absorbs light within the retina. Photoexcitation of rhodopsin causes the cytoplasmic surface of the protein to become catalytically active. In the active state, rhodopsin activates transducin, a GTP binding protein. Once activated, transducin promotes the hydrolysis of cGMP by phosphodiesterase (PDE). The decrease of intracellular cGMP concentrations causes the ion channels within the outer segment of the rod or cone to close, thus causing membrane hyperpolarization and, eventually, signal transmission. Rhodopsin activity is believed to be shut off by phosphorylation followed by binding of the soluble proteins arrestin.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products


Our Abpromise guarantee covers the use of ab3424 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 38 kDa).Can be blocked with Rhodopsin peptide (ab4967).

By Western blot, this antibody detects an ~36 kDa protein representing rhodopsin from bovine outer rod segment extracts.

IHC-Fr Use at an assay dependent concentration. PubMed: 22743318


  • FunctionPhotoreceptor required for image-forming vision at low light intensity. Required for photoreceptor cell viability after birth. Light-induced isomerization of 11-cis to all-trans retinal triggers a conformational change leading to G-protein activation and release of all-trans retinal.
  • Tissue specificityRod shaped photoreceptor cells which mediates vision in dim light.
  • Involvement in diseaseRetinitis pigmentosa 4
    Night blindness, congenital stationary, autosomal dominant 1
  • Sequence similaritiesBelongs to the G-protein coupled receptor 1 family. Opsin subfamily.
  • Post-translational
    Phosphorylated on some or all of the serine and threonine residues present in the C-terminal region.
    Contains one covalently linked retinal chromophore.
  • Cellular localizationMembrane. Synthesized in the inner segment (IS) of rod photoreceptor cells before vectorial transport to the rod outer segment (OS) photosensory cilia.
  • Information by UniProt
  • Database links
  • Alternative names
    • CSNBAD1 antibody
    • MGC138309 antibody
    • MGC138311 antibody
    • OPN 2 antibody
    • OPN2 antibody
    • opsd antibody
    • OPSD_HUMAN antibody
    • opsin 2 antibody
    • Opsin 2 rod pigment antibody
    • Opsin-2 antibody
    • Opsin2 antibody
    • Retinitis Pigmentosa 4 antibody
    • Retinitis pigmentosa 4 autosomal dominant antibody
    • RHO antibody
    • Rhodopsin antibody
    • RP 4 antibody
    • RP4 antibody
    see all

Anti-Rhodopsin antibody images

  • Immunohistochemistry (Frozen sections) image of anti-Rhodopsin antibody (ab3424) staining on a Zebrafish eye. This tissue section was stained with anti-Rhodopsin antibody ab3424 (green) and anti-Rhodopsin monoclonal antibody ab5417 (red) for 16 hours at 4°C. Nuclei were stained with DAPI (blue). This image suggests that ab3424 (like ab5417) may detect Red Opsin and not Rhodopsin in the Zebrafish. For more information please see Yin J. et al., 2012.

    See Abreview

References for Anti-Rhodopsin antibody (ab3424)

This product has been referenced in:
  • Yin J  et al. The 1D4 antibody labels outer segments of long double cone but not rod photoreceptors in zebrafish. Invest Ophthalmol Vis Sci 53:4943-51 (2012). IHC-Fr ; Zebrafish . Read more (PubMed: 22743318) »

See 1 Publication for this product

Product Wall

Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Rat Tissue sections (Brain)
Antigen retrieval step None
Permeabilization Yes - 0.4% triton
Specification Brain
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Fixative Paraformaldehyde

Brian Maunze

Verified customer

Submitted Jul 20 2015

Application Immunohistochemistry (Frozen sections)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Sample Zebrafish Tissue sections (Long double cone photoreceptor cells in zebrafish)
Specification Long double cone photoreceptor cells in zebrafish
Permeabilization Yes - PBST
Fixative Paraformaldehyde

Dr. Jun Yin

Verified customer

Submitted Jul 04 2013