Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferConstituents: 0.1% BSA, 99% PBS
Concentration information loading...
PurityImmunogen affinity purified
Primary antibody notesVision involves the conversion of light into electrochemical signals that are processed by the retina and subsequently sent to and interpreted by the brain. The process of converting light to an electrochemical signal begins when the membrane-bound protein rhodopsin absorbs light within the retina. Photoexcitation of rhodopsin causes the cytoplasmic surface of the protein to become catalytically active. In the active state, rhodopsin activates transducin, a GTP binding protein. Once activated, transducin promotes the hydrolysis of cGMP by phosphodiesterase (PDE). The decrease of intracellular cGMP concentrations causes the ion channels within the outer segment of the rod or cone to close, thus causing membrane hyperpolarization and, eventually, signal transmission. Rhodopsin activity is believed to be shut off by phosphorylation followed by binding of the soluble proteins arrestin.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 2 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 38 kDa).Can be blocked with Rhodopsin peptide (ab4967).
By Western blot, this antibody detects an ~36 kDa protein representing rhodopsin from bovine outer rod segment extracts.
Use at an assay dependent concentration. PubMed: 22743318
FunctionPhotoreceptor required for image-forming vision at low light intensity. Required for photoreceptor cell viability after birth. Light-induced isomerization of 11-cis to all-trans retinal triggers a conformational change leading to G-protein activation and release of all-trans retinal.
Tissue specificityRod shaped photoreceptor cells which mediates vision in dim light.
Involvement in diseaseRetinitis pigmentosa 4 Night blindness, congenital stationary, autosomal dominant 1
Sequence similaritiesBelongs to the G-protein coupled receptor 1 family. Opsin subfamily.
Post-translational modificationsPhosphorylated on some or all of the serine and threonine residues present in the C-terminal region. Contains one covalently linked retinal chromophore.
Cellular localizationMembrane. Synthesized in the inner segment (IS) of rod photoreceptor cells before vectorial transport to the rod outer segment (OS) photosensory cilia.
Immunohistochemistry (Frozen sections) - Anti-Rhodopsin antibody (ab3424)This image is courtesy of an abreview from Dr. Jun Yin.
Immunohistochemistry (Frozen sections) image of anti-Rhodopsin antibody (ab3424) staining on a Zebrafish eye. This tissue section was stained with anti-Rhodopsin antibody ab3424 (green) and anti-Rhodopsin monoclonal antibody ab5417 (red) for 16 hours at 4°C. Nuclei were stained with DAPI (blue). This image suggests that ab3424 (like ab5417) may detect Red Opsin and not Rhodopsin in the Zebrafish. For more information please see Yin J. et al., 2012.
Yin J et al. The 1D4 antibody labels outer segments of long double cone but not rod photoreceptors in zebrafish. Invest Ophthalmol Vis Sci53:4943-51 (2012).
Read more (PubMed: 22743318) »