Recombinant
RabMAb

Anti-Rhodopsin antibody [EPR7996] (ab155097)

Overview

  • Product name
    Anti-Rhodopsin antibody [EPR7996]
    See all Rhodopsin primary antibodies
  • Description
    Rabbit monoclonal [EPR7996] to Rhodopsin
  • Tested applications
    Suitable for: WB, IHC-Pmore details
    Unsuitable for: ICC or IP
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Rhodopsin aa 300-400.

  • Positive control
    • Rat eyeball lysate; Human retina tissue.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab155097 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 39 kDa.
IHC-P 1/50 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for ICC or IP.
  • Target

    • Function
      Photoreceptor required for image-forming vision at low light intensity. Required for photoreceptor cell viability after birth. Light-induced isomerization of 11-cis to all-trans retinal triggers a conformational change leading to G-protein activation and release of all-trans retinal.
    • Tissue specificity
      Rod shaped photoreceptor cells which mediates vision in dim light.
    • Involvement in disease
      Retinitis pigmentosa 4
      Night blindness, congenital stationary, autosomal dominant 1
    • Sequence similarities
      Belongs to the G-protein coupled receptor 1 family. Opsin subfamily.
    • Post-translational
      modifications
      Phosphorylated on some or all of the serine and threonine residues present in the C-terminal region.
      Contains one covalently linked retinal chromophore.
    • Cellular localization
      Membrane. Synthesized in the inner segment (IS) of rod photoreceptor cells before vectorial transport to the rod outer segment (OS) photosensory cilia.
    • Information by UniProt
    • Database links
    • Alternative names
      • CSNBAD1 antibody
      • MGC138309 antibody
      • MGC138311 antibody
      • OPN 2 antibody
      • OPN2 antibody
      • opsd antibody
      • OPSD_HUMAN antibody
      • opsin 2 antibody
      • Opsin 2 rod pigment antibody
      • Opsin-2 antibody
      • Opsin2 antibody
      • Retinitis Pigmentosa 4 antibody
      • Retinitis pigmentosa 4 autosomal dominant antibody
      • RHO antibody
      • Rhodopsin antibody
      • RP 4 antibody
      • RP4 antibody
      see all

    Images

    • Anti-Rhodopsin antibody [EPR7996] (ab155097) at 1/1000 dilution + Rat eyeball lysate at 10 µg

      Secondary
      Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size : 39 kDa
    • Immunohistochemical analysis of paraffin-embedded Human retina tissue labeling Rhodopsin with ab155097 at 1/50 dilution.
    • Immunohistochemical analysis of paraffin embedded Human ovarian carcinoma tissue using ab155097 showing -ve staining.

    • Immunohistochemical analysis of paraffin embedded Human normal pancreas tissue using ab155097 showing -ve staining.

    • Immunohistochemical analysis of paraffin embedded Human hepatocellular carcinoma tissue using ab155097 showing -ve staining.

    • Immunohistochemical analysis of paraffin embedded Human normal colon tissue using ab155097 showing -ve staining.

    References

    ab155097 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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