1/50 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Is unsuitable for ICC or IP.
Photoreceptor required for image-forming vision at low light intensity. Required for photoreceptor cell viability after birth. Light-induced isomerization of 11-cis to all-trans retinal triggers a conformational change leading to G-protein activation and release of all-trans retinal.
Rod shaped photoreceptor cells which mediates vision in dim light.
Involvement in disease
Retinitis pigmentosa 4 Night blindness, congenital stationary, autosomal dominant 1
Belongs to the G-protein coupled receptor 1 family. Opsin subfamily.
Phosphorylated on some or all of the serine and threonine residues present in the C-terminal region. Contains one covalently linked retinal chromophore.
Membrane. Synthesized in the inner segment (IS) of rod photoreceptor cells before vectorial transport to the rod outer segment (OS) photosensory cilia.