Membrane preparation from adult rat retina.
Rhodopsin is a glycoprotein which is a visual pigment in mammals, birds, amphibians, and reptiles. The rhodopsin protein is present on the photoreceptor cells in the retina.
Our Abpromise guarantee covers the use of ab3267 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration.|
|Flow Cyt||Use at an assay dependent concentration.
ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|IHC-P||1/25 - 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Staining protocol - boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10 - 20 min followed by cooling at RT for 20 min.
|WB||Use at an assay dependent concentration. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa).
Blot incubation - 2hrs at RT
|IP||Use at an assay dependent concentration. PubMed: 18974094|
Image from Chucair-Elliott AJ et al., J Biol Chem 287:24092-102 (2012); Fig 1.; doi: 10.1074/jbc.M112.378240; July 13, 2012, The Journal of Biological Chemistry, 287, 24092-24102.