Overview

  • Product nameAnti-Rhodopsin antibody [RET-P1]
    See all Rhodopsin primary antibodies
  • Description
    Mouse monoclonal [RET-P1] to Rhodopsin
  • Specificityab81702 specifically labels the axons and synaptic pedicles of the rods.
  • Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Membrane preparation from adult rat retina.

  • Positive control
    • Brain or retina.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 10mM PBS, pH 7.4
  • Concentration information loading...
  • PurityProtein G purified
  • Purification notesPurified from ascites fluid by Protein G chromatography.
  • ClonalityMonoclonal
  • Clone numberRET-P1
  • IsotypeIgG1
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab81702 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IHC-P
ICC/IF
  • Application notesICC/IF: Use at an assay dependent dilution.
    IHC-P: 1/25 - 1/50 for 60 minutes at room temperature. Staining of formalin fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at room temperature for 20 minutes.
    WB: Use at a concentration of 1 - 2 µg/ml for 2 hours at room temperature. Predicted molecular weight: 39 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionPhotoreceptor required for image-forming vision at low light intensity. Required for photoreceptor cell viability after birth. Light-induced isomerization of 11-cis to all-trans retinal triggers a conformational change leading to G-protein activation and release of all-trans retinal.
    • Tissue specificityRod shaped photoreceptor cells which mediates vision in dim light.
    • Involvement in diseaseRetinitis pigmentosa 4
      Night blindness, congenital stationary, autosomal dominant 1
    • Sequence similaritiesBelongs to the G-protein coupled receptor 1 family. Opsin subfamily.
    • Post-translational
      modifications
      Phosphorylated on some or all of the serine and threonine residues present in the C-terminal region.
      Contains one covalently linked retinal chromophore.
    • Cellular localizationMembrane. Synthesized in the inner segment (IS) of rod photoreceptor cells before vectorial transport to the rod outer segment (OS) photosensory cilia.
    • Information by UniProt
    • Database links
    • Alternative names
      • CSNBAD1 antibody
      • MGC138309 antibody
      • MGC138311 antibody
      • OPN 2 antibody
      • OPN2 antibody
      • opsd antibody
      • OPSD_HUMAN antibody
      • opsin 2 antibody
      • Opsin 2 rod pigment antibody
      • Opsin-2 antibody
      • Opsin2 antibody
      • Retinitis Pigmentosa 4 antibody
      • Retinitis pigmentosa 4 autosomal dominant antibody
      • RHO antibody
      • Rhodopsin antibody
      • RP 4 antibody
      • RP4 antibody
      see all

    Anti-Rhodopsin antibody [RET-P1] images

    • Formalin fixed, paraffin embedded human brain tissue stained with anti Rhodopsin (form of ab81702 containing BSA and azide) at a 1/25 dilution. Peroxidase conjugate and AEC chromogen were used. Note cytoplasmic staining of neurons.
    • anti Rhodopsin (form of ab81702 containing BSA and azide) at 2 µg/ml + human cells

      Predicted band size : 39 kDa
      Observed band size : 39 kDa

    References for Anti-Rhodopsin antibody [RET-P1] (ab81702)

    ab81702 has not yet been referenced specifically in any publications.

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    There are currently no Abreviews or Questions for ab81702.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"