The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesICC/IF: Use at an assay dependent dilution.
IHC-P: 1/25 - 1/50 for 60 minutes at room temperature. Staining of formalin fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at room temperature for 20 minutes.
WB: Use at a concentration of 1 - 2 µg/ml for 2 hours at room temperature. Predicted molecular weight: 39 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionPhotoreceptor required for image-forming vision at low light intensity. Required for photoreceptor cell viability after birth. Light-induced isomerization of 11-cis to all-trans retinal triggers a conformational change leading to G-protein activation and release of all-trans retinal.
Tissue specificityRod shaped photoreceptor cells which mediates vision in dim light.
Involvement in diseaseRetinitis pigmentosa 4 Night blindness, congenital stationary, autosomal dominant 1
Sequence similaritiesBelongs to the G-protein coupled receptor 1 family. Opsin subfamily.
Post-translational modificationsPhosphorylated on some or all of the serine and threonine residues present in the C-terminal region. Contains one covalently linked retinal chromophore.
Cellular localizationMembrane. Synthesized in the inner segment (IS) of rod photoreceptor cells before vectorial transport to the rod outer segment (OS) photosensory cilia.
Formalin fixed, paraffin embedded human brain tissue stained with anti Rhodopsin (form of ab81702 containing BSA and azide) at a 1/25 dilution. Peroxidase conjugate and AEC chromogen were used. Note cytoplasmic staining of neurons.
Western blot - Rhodopsin antibody [RET-P1] - BSA and Azide free (ab81702)
anti Rhodopsin (form of ab81702 containing BSA and azide) at 2 µg/ml + human cells
Predicted band size : 39 kDa Observed band size : 39 kDa
References for Anti-Rhodopsin antibody [RET-P1] (ab81702)
has not yet been referenced specifically in any publications.
Publishing research using ab81702? Please let us know so that we can cite the reference in this datasheet.
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