Anti-Rhodopsin (phospho S334) antibody (ab59224)

Overview

  • Product nameAnti-Rhodopsin (phospho S334) antibody
    See all Rhodopsin primary antibodies
  • Description
    Rabbit polyclonal to Rhodopsin (phospho S334)
  • Specificityab59224 detects endogenous levels of Rhodopsin only when phosphorylated at serine 334.
  • Tested applicationsSuitable for: ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic phosphopeptide derived from human Rhodopsin around the phosphorylation site of serine 334 (D-A-SP-A-T).

  • Positive control
    • Human brain tissue.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesab59224 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab59224 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/5000.
IHC-P 1/50 - 1/100.

Target

  • FunctionPhotoreceptor required for image-forming vision at low light intensity. Required for photoreceptor cell viability after birth. Light-induced isomerization of 11-cis to all-trans retinal triggers a conformational change leading to G-protein activation and release of all-trans retinal.
  • Tissue specificityRod shaped photoreceptor cells which mediates vision in dim light.
  • Involvement in diseaseRetinitis pigmentosa 4
    Night blindness, congenital stationary, autosomal dominant 1
  • Sequence similaritiesBelongs to the G-protein coupled receptor 1 family. Opsin subfamily.
  • Post-translational
    modifications
    Phosphorylated on some or all of the serine and threonine residues present in the C-terminal region.
    Contains one covalently linked retinal chromophore.
  • Cellular localizationMembrane. Synthesized in the inner segment (IS) of rod photoreceptor cells before vectorial transport to the rod outer segment (OS) photosensory cilia.
  • Information by UniProt
  • Database links
  • Alternative names
    • CSNBAD1 antibody
    • MGC138309 antibody
    • MGC138311 antibody
    • OPN 2 antibody
    • OPN2 antibody
    • opsd antibody
    • OPSD_HUMAN antibody
    • opsin 2 antibody
    • Opsin 2 rod pigment antibody
    • Opsin-2 antibody
    • Opsin2 antibody
    • Retinitis Pigmentosa 4 antibody
    • Retinitis pigmentosa 4 autosomal dominant antibody
    • RHO antibody
    • Rhodopsin antibody
    • RP 4 antibody
    • RP4 antibody
    see all

Anti-Rhodopsin (phospho S334) antibody images

  • ab59224 (1/50 dilution staining) Rhodopsin in human brain tissue by Immunohistochemistry (Paraffin section); in the absence or presence of the immunising peptide.

References for Anti-Rhodopsin (phospho S334) antibody (ab59224)

ab59224 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab59224.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"