Purification notesab59224 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against
non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/50 - 1/100.
FunctionPhotoreceptor required for image-forming vision at low light intensity. Required for photoreceptor cell viability after birth. Light-induced isomerization of 11-cis to all-trans retinal triggers a conformational change leading to G-protein activation and release of all-trans retinal.
Tissue specificityRod shaped photoreceptor cells which mediates vision in dim light.
Involvement in diseaseRetinitis pigmentosa 4 Night blindness, congenital stationary, autosomal dominant 1
Sequence similaritiesBelongs to the G-protein coupled receptor 1 family. Opsin subfamily.
Post-translational modificationsPhosphorylated on some or all of the serine and threonine residues present in the C-terminal region. Contains one covalently linked retinal chromophore.
Cellular localizationMembrane. Synthesized in the inner segment (IS) of rod photoreceptor cells before vectorial transport to the rod outer segment (OS) photosensory cilia.