Human Predicted to work with:
Mouse, Rat, Hamster, Cow, Pig, Duck
Recombinant fragment with tag (MW of the tag alone is 26 KDa): RHKWHPEVCH HCPDVPILLV GTKKDLRAQP DTLRRLKEQG QAPITPQQGQ ALAKQIHAV RYLECSALQQ DGVKEVFAEA VRAVLNPTPI KRGRSCILL, corresponding to amino acids 94-192 of Human RHOG. Note: the sequence provided does not include that of the tag.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesELISA: Use at an assay dependent dilution.
sELISA: Use at an assay dependent dilution.
WB: 1/500 - 1/1000. Predicted molecular weight: 21 kDa.
This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionRequired for the formation of membrane ruffles during macropinocytosis. Required for the formation of cup-like structures during trans-endothelial migration of leukocytes. In case of Salmonella enterica infection, activated by SopB and ARHGEF26/SGEF, which induces cytoskeleton rearrangements and promotes bacterial entry.
Sequence similaritiesBelongs to the small GTPase superfamily. Rho family.