Overview

  • Product nameAnti-RHOG antibody [2E6]
    See all RHOG primary antibodies
  • Description
    Mouse monoclonal [2E6] to RHOG
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Hamster, Cow, Pig, Duck
  • Immunogen

    Recombinant fragment with tag (MW of the tag alone is 26 KDa): RHKWHPEVCH HCPDVPILLV GTKKDLRAQP DTLRRLKEQG QAPITPQQGQ ALAKQIHAV RYLECSALQQ DGVKEVFAEA VRAVLNPTPI KRGRSCILL, corresponding to amino acids 94-192 of Human RHOG. Note: the sequence provided does not include that of the tag.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityMonoclonal
  • Clone number2E6
  • IsotypeIgG2a
  • Light chain typekappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab76508 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
  • Application notesELISA: Use at an assay dependent dilution.
    sELISA: Use at an assay dependent dilution.
    WB: 1/500 - 1/1000. Predicted molecular weight: 21 kDa.

    This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionRequired for the formation of membrane ruffles during macropinocytosis. Required for the formation of cup-like structures during trans-endothelial migration of leukocytes. In case of Salmonella enterica infection, activated by SopB and ARHGEF26/SGEF, which induces cytoskeleton rearrangements and promotes bacterial entry.
    • Sequence similaritiesBelongs to the small GTPase superfamily. Rho family.
    • Cellular localizationCell membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • ARHG antibody
      • Ras homolog family member G antibody
      • Rho G antibody
      • Rho related GTP binding protein RhoG precursor antibody
      • Rho-related GTP-binding protein RhoG antibody
      • RHOG antibody
      • RHOG_HUMAN antibody
      see all

    Anti-RHOG antibody [2E6] images

    • Anti-RHOG antibody [2E6] (ab76508) at 1/500 dilution + immunogen at 0.2 µg

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution

      Predicted band size : 21 kDa
      Observed band size : 37 kDa (why is the actual band size different from the predicted?)
    • Detection limit for recombinant tagged RHOG is approximately 1ng/ml when used as a capture antibody at 1ug/well.

    References for Anti-RHOG antibody [2E6] (ab76508)

    ab76508 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab76508.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"