Overview

  • Product nameAnti-RhoGAP antibody
    See all RhoGAP primary antibodies
  • Description
    Rabbit polyclonal to RhoGAP
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    KLH conjugated synthetic peptide selected from the center region of Human RhoGAP (NP_001164.2).

  • Positive control
    • 293 cell line lysate; Mouse cerebellum tissue lysates

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesab93375 is purified through a protein A column, followed by peptide affinity purification.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab93375 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
  • Application notesELISA: 1/1000.
    WB: 1/100 - 1/500. Predicted molecular weight: 171 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceThe p190 RhoGAP protein is associated with p120 RasGAP in growth-factor stimulated and tyrosine kinase transformed cells. It functions as a GTPase - activating protein (GAP) for Rho and Rac family proteins, which are involved in regulating cytoskeletal actin and membrane ruffling. The antibody appears to couple signal transduction via Ras and Rho through its association with RasGAP. The presence of two adjacent SH2 domains in the p21 RAS GAP indicates that GAP might interact directly with tyrosine kinases. p190B can be directly phosphorylated on a single identified tyrosine residue by the activated insulin and IGF-1 receptors. This phosphorylation causes p190B to translocate to the lipid rafts of the plasma membrane, where it can facilitate the inactivation of the Rho GTPase. P190B also has been shown to be tyrosine phosphorylated by cSrc and vSrc. Additionally the GAP domain of p190B is shown to attenuate signal transducing activity of Rac, Rho and CDC42.
    • Cellular localizationCytoplasmic
    • Database links
    • Alternative names
      • ARHGAP 5 antibody
      • ARHGAP5 antibody
      • p190 B antibody
      • p190 RhoGAP antibody
      • Rho GTPase activating protein 5 antibody
      • RhoGAP 5 antibody
      • RhoGAP antibody
      • RhoGAP5 antibody
      see all

    Anti-RhoGAP antibody images

    • All lanes : Anti-RhoGAP antibody (ab93375) at 1/100 dilution

      Lane 1 : 293 cell line lysate
      Lane 2 : Mouse cerebellum tissue lysate

      Lysates/proteins at 35 µg per lane.


      Predicted band size : 171 kDa

    References for Anti-RhoGAP antibody (ab93375)

    ab93375 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab93375.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"