Overview

  • Product nameAnti-RhoGDI antibody [1F2]
    See all RhoGDI primary antibodies
  • Description
    Mouse monoclonal [1F2] to RhoGDI
  • Tested applicationsSuitable for: WB, Flow Cytmore details
  • Species reactivity
    Reacts with: Rat, Dog, Human, Monkey
  • Immunogen

    Recombinant full length protein of Human RhoGDI produced in HEK293T cells (NP_004300).

  • Positive control
    • RhoGDI traansfected HEK293T cell lysate, HepG2, HeLa, HT29, A549, COS7, Jurkat, MDCK, PC12, and MCF7 cell extracts, RhoGDI traansfected HEK293T cells, Hela and Jurkat cells
  • General notesDilute in PBS (pH7.3) before use.

Properties

Applications

Our Abpromise guarantee covers the use of ab118159 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/200 - 1/1000. Predicted molecular weight: 23 kDa.
Flow Cyt 1/100. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • FunctionRegulates the GDP/GTP exchange reaction of the Rho proteins by inhibiting the dissociation of GDP from them, and the subsequent binding of GTP to them.
  • Sequence similaritiesBelongs to the Rho GDI family.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • ARHGDIA antibody
    • fa96g11 antibody
    • GDIA 1 antibody
    • GDIA1 antibody
    • GDIR1_HUMAN antibody
    • MGC117248 antibody
    • NPHS8 antibody
    • Rho GDI 1 antibody
    • Rho GDI alpha antibody
    • Rho GDI antibody
    • Rho GDP dissociation inhibitor (GDI) alpha antibody
    • Rho GDP dissociation inhibitor 1 antibody
    • Rho GDP dissociation inhibitor alpha antibody
    • Rho GDP-dissociation inhibitor 1 antibody
    • Rho-GDI alpha antibody
    • RhoGDI 1 antibody
    • RhoGDI alpha antibody
    • RHOGDI antibody
    • RhoGDI1 antibody
    • wu:fa96g11 antibody
    • zgc:55554 antibody
    • zgc:77681 antibody
    see all

Anti-RhoGDI antibody [1F2] images

  • All lanes : Anti-RhoGDI antibody [1F2] (ab118159) at 1/200 dilution

    Lane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY control
    Lane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY RhoGDI cDNA

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 23 kDa
    HEK293T cell lysates were generated from transient transfection of the cDNA clone RC213934
  • All lanes : Anti-RhoGDI antibody [1F2] (ab118159) at 1/200 dilution

    Lane 1 : HepG2 cell extract
    Lane 2 : HeLa cell extract
    Lane 3 : HT29 cell extract
    Lane 4 : A549 cell extract
    Lane 5 : COS7 cell extract
    Lane 6 : Jurkat cell extract
    Lane 7 : MDCK cell extract
    Lane 8 : PC12 cell extract
    Lane 9 : MCF7 cell extract

    Lysates/proteins at 35 µg per lane.


    Predicted band size : 23 kDa
    HEK293T cell lysates were generated from transient transfection of the cDNA clone RC213934
  • HEK293T cells transfected with either RhoGDI overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by ab118159 at a dilution of 1/100, and then analyzed by flow cytometry.
  • Flow cytometric Analysis of Hela cells, using ab118159 at a dilution of 1/100 (Red), compared to a nonspecific negative control antibody (Blue).
  • Flow cytometric Analysis of Jurkat cells, using ab118159 at a dilution of 1/100 (Red), compared to a nonspecific negative control antibody (Blue).

References for Anti-RhoGDI antibody [1F2] (ab118159)

This product has been referenced in:
  • Saxena R  et al. Proteomic profiling of SupT1 cells reveal modulation of host proteins by HIV-1 Nef variants. PLoS One 10:e0122994 (2015). WB, IF ; Human . Read more (PubMed: 25874870) »

See 1 Publication for this product

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