Recombinant
RabMAb

Anti-RhoGDI antibody [EPR3772] (ab108977)

Overview

  • Product name
    Anti-RhoGDI antibody [EPR3772]
    See all RhoGDI primary antibodies
  • Description
    Rabbit monoclonal [EPR3772] to RhoGDI
  • Tested applications
    Suitable for: WB, ICCmore details
    Unsuitable for: Flow Cyt,IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues in Human RhoGDI.

  • Positive control
    • Jurkat, HeLa, HL-60 and NIH/3T3 cell lysates
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    A trial size is available to purchase for this antibody.

    Alternative versions available:

    Anti-RhoGDI antibody (HRP) [EPR3772] (ab200183)

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab108977 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Detects a band of approximately 26 kDa (predicted molecular weight: 23 kDa).
ICC 1/50 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt,IHC-P or IP.
  • Target

    • Function
      Regulates the GDP/GTP exchange reaction of the Rho proteins by inhibiting the dissociation of GDP from them, and the subsequent binding of GTP to them.
    • Sequence similarities
      Belongs to the Rho GDI family.
    • Cellular localization
      Cytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • ARHGDIA antibody
      • fa96g11 antibody
      • GDIA 1 antibody
      • GDIA1 antibody
      • GDIR1_HUMAN antibody
      • MGC117248 antibody
      • NPHS8 antibody
      • Rho GDI 1 antibody
      • Rho GDI alpha antibody
      • Rho GDI antibody
      • Rho GDP dissociation inhibitor (GDI) alpha antibody
      • Rho GDP dissociation inhibitor 1 antibody
      • Rho GDP dissociation inhibitor alpha antibody
      • Rho GDP-dissociation inhibitor 1 antibody
      • Rho-GDI alpha antibody
      • RhoGDI 1 antibody
      • RhoGDI alpha antibody
      • RHOGDI antibody
      • RhoGDI1 antibody
      • wu:fa96g11 antibody
      • zgc:55554 antibody
      • zgc:77681 antibody
      see all

    Anti-RhoGDI antibody [EPR3772] images

    • All lanes : Anti-RhoGDI antibody [EPR3772] (ab108977) at 1/1000 dilution

      Lane 1 : Jurkat cell lysates
      Lane 2 : HeLa cell lysates
      Lane 3 : HL-60 cell lysates
      Lane 4 : NIH/3T3 cell lysates

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 23 kDa
      Observed band size : 26 kDa (why is the actual band size different from the predicted?)

    References for Anti-RhoGDI antibody [EPR3772] (ab108977)

    This product has been referenced in:
    • Kempf SJ  et al. The cognitive defects of neonatally irradiated mice are accompanied by changed synaptic plasticity, adult neurogenesis and neuroinflammation. Mol Neurodegener 9:57 (2014). WB . Read more (PubMed: 25515237) »
    • Yentrapalli R  et al. The PI3K/Akt/mTOR pathway is implicated in the premature senescence of primary human endothelial cells exposed to chronic radiation. PLoS One 8:e70024 (2013). Human . Read more (PubMed: 23936371) »

    See all 2 Publications for this product

    Product Wall

    Thank you for your email.

    Unfortunately the order is out of Abpromise guarantee period. The order was placed on 17th January 2012, which means it was under 6 month guarantee period until 16th June however the complaint was submitted in August ...

    Read More

    Thanks you for your email. I apologize for the delay.

    We unfortunately are unable to find reason why the activity of this antibody is dropping. Also we haven't received any other quality complaint for this antibody so I am sorry we will not be...

    Read More

    Thank you very much for sending the information.

    In order to understand the problem better could you provide details how this antibody was stored?

    Was the antibody degradation gradual or it just stopped working after 6 months?
    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up