Overview

  • Product nameAnti-RhoGDI antibody [OTI1F2]
    See all RhoGDI primary antibodies
  • Description
    Mouse monoclonal [OTI1F2] to RhoGDI
  • Tested applicationsSuitable for: WB, Flow Cytmore details
  • Species reactivity
    Reacts with: Rat, Dog, Human, Monkey
  • Immunogen

    Recombinant full length protein of Human RhoGDI produced in HEK293T cells (NP_004300).

  • Positive control
    • RhoGDI traansfected HEK293T cell lysate, HepG2, HeLa, HT29, A549, COS7, Jurkat, MDCK, PC12, and MCF7 cell extracts, RhoGDI traansfected HEK293T cells, Hela and Jurkat cells
  • General notes

    Dilute in PBS (pH7.3) before use.

    The clone number has been updated from 1F2 to OTI1F2, both clone numbers name the same clone.

Properties

Applications

Our Abpromise guarantee covers the use of ab118159 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/200 - 1/1000. Predicted molecular weight: 23 kDa.
Flow Cyt 1/100. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • FunctionRegulates the GDP/GTP exchange reaction of the Rho proteins by inhibiting the dissociation of GDP from them, and the subsequent binding of GTP to them.
  • Sequence similaritiesBelongs to the Rho GDI family.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • ARHGDIA antibody
    • fa96g11 antibody
    • GDIA 1 antibody
    • GDIA1 antibody
    • GDIR1_HUMAN antibody
    • MGC117248 antibody
    • NPHS8 antibody
    • Rho GDI 1 antibody
    • Rho GDI alpha antibody
    • Rho GDI antibody
    • Rho GDP dissociation inhibitor (GDI) alpha antibody
    • Rho GDP dissociation inhibitor 1 antibody
    • Rho GDP dissociation inhibitor alpha antibody
    • Rho GDP-dissociation inhibitor 1 antibody
    • Rho-GDI alpha antibody
    • RhoGDI 1 antibody
    • RhoGDI alpha antibody
    • RHOGDI antibody
    • RhoGDI1 antibody
    • wu:fa96g11 antibody
    • zgc:55554 antibody
    • zgc:77681 antibody
    see all

Anti-RhoGDI antibody [OTI1F2] images

  • All lanes : Anti-RhoGDI antibody [OTI1F2] (ab118159) at 1/200 dilution

    Lane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY control
    Lane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY RhoGDI cDNA

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 23 kDa
    HEK293T cell lysates were generated from transient transfection of the cDNA clone RC213934
  • All lanes : Anti-RhoGDI antibody [OTI1F2] (ab118159) at 1/200 dilution

    Lane 1 : HepG2 cell extract
    Lane 2 : HeLa cell extract
    Lane 3 : HT29 cell extract
    Lane 4 : A549 cell extract
    Lane 5 : COS7 cell extract
    Lane 6 : Jurkat cell extract
    Lane 7 : MDCK cell extract
    Lane 8 : PC12 cell extract
    Lane 9 : MCF7 cell extract

    Lysates/proteins at 35 µg per lane.


    Predicted band size : 23 kDa
    HEK293T cell lysates were generated from transient transfection of the cDNA clone RC213934
  • HEK293T cells transfected with either RhoGDI overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by ab118159 at a dilution of 1/100, and then analyzed by flow cytometry.
  • Flow cytometric Analysis of Hela cells, using ab118159 at a dilution of 1/100 (Red), compared to a nonspecific negative control antibody (Blue).
  • Flow cytometric Analysis of Jurkat cells, using ab118159 at a dilution of 1/100 (Red), compared to a nonspecific negative control antibody (Blue).

References for Anti-RhoGDI antibody [OTI1F2] (ab118159)

This product has been referenced in:
  • Saxena R  et al. Proteomic profiling of SupT1 cells reveal modulation of host proteins by HIV-1 Nef variants. PLoS One 10:e0122994 (2015). WB, IF ; Human . Read more (PubMed: 25874870) »

See 1 Publication for this product

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