Overview

  • Product name
    Anti-RhoGDI (phospho S174) antibody
    See all RhoGDI primary antibodies
  • Description
    Rabbit polyclonal to RhoGDI (phospho S174)
  • Specificity
    The antibody detects endogenous levels of RhoGDI only when phosphorylated at serine 174.
  • Tested applications
    Suitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthesized phosphopeptide derived from human RhoGDI around the phosphorylation site of serine 174 (R-G-SP-Y-S).

  • Positive control
    • IHC-P: Human cervix carcinoma tissue. WB: HUVEC cells treated with EGF.

Properties

Applications

Our Abpromise guarantee covers the use of ab74142 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 23 kDa.
ELISA 1/20000.
IHC-P 1/50 - 1/100.

Target

  • Function
    Regulates the GDP/GTP exchange reaction of the Rho proteins by inhibiting the dissociation of GDP from them, and the subsequent binding of GTP to them.
  • Sequence similarities
    Belongs to the Rho GDI family.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • ARHGDIA antibody
    • fa96g11 antibody
    • GDIA 1 antibody
    • GDIA1 antibody
    • GDIR1_HUMAN antibody
    • MGC117248 antibody
    • NPHS8 antibody
    • Rho GDI 1 antibody
    • Rho GDI alpha antibody
    • Rho GDI antibody
    • Rho GDP dissociation inhibitor (GDI) alpha antibody
    • Rho GDP dissociation inhibitor 1 antibody
    • Rho GDP dissociation inhibitor alpha antibody
    • Rho GDP-dissociation inhibitor 1 antibody
    • Rho-GDI alpha antibody
    • RhoGDI 1 antibody
    • RhoGDI alpha antibody
    • RHOGDI antibody
    • RhoGDI1 antibody
    • wu:fa96g11 antibody
    • zgc:55554 antibody
    • zgc:77681 antibody
    see all

Images

  • All lanes : Anti-RhoGDI (phospho S174) antibody (ab74142) at 1/500 dilution

    Lane 1 : HUVEC cells treated with EGF (200ng/ml, 30mins)
    Lane 2 : HUVEC cells treated with EGF (200ng/ml, 30mins) with phosphopeptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 23 kDa
    Observed band size : 23 kDa
    Additional bands at : 105 kDa,60 kDa. We are unsure as to the identity of these extra bands.
  • Human cervix carcinoma tissue stained with ab74142 (1/50 dilution).
    1: No phosphopeptide
    2: Phosphopeptide

References

This product has been referenced in:
  • Oishi A  et al. Regulation of RhoA Signaling by the cAMP-dependent Phosphorylation of RhoGDIa. J Biol Chem 287:38705-15 (2012). WB . Read more (PubMed: 23012358) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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