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Storage instructions Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg 2+ and Ca 2++), 150mM Sodium chloride, pH 7.4
Concentration information loading...
Purity Immunogen affinity purified
Purification notes The antibody was purified by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Predicted molecular weight: 23 kDa.
1/50 - 1/100.
Function Regulates the GDP/GTP exchange reaction of the Rho proteins by inhibiting the dissociation of GDP from them, and the subsequent binding of GTP to them.
Sequence similarities Belongs to the Rho GDI family.
Cellular localization Cytoplasm.
Information by UniProt
GDIA 1 antibody
Anti-RhoGDI (phospho S174) antibody images
Western blot - RhoGDI (phospho S174) antibody (ab74142)
All lanes : Anti-RhoGDI (phospho S174) antibody (ab74142) at 1/500 dilution Lane 1 : HUVEC cells treated with EGF (200ng/ml, 30mins) Lane 2 : HUVEC cells treated with EGF (200ng/ml, 30mins) with phosphopeptide at 10 µg Lysates/proteins at 30 µg per lane. Predicted band size : 23 kDa Observed band size : 23 kDa Additional bands at : 105 kDa,60 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - RhoGDI (phospho S174) antibody (ab74142)
Human cervix carcinoma tissue stained with ab74142 (1/50 dilution).
1: No phosphopeptide
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