Anti-RHOH antibody (ab118507)
Key features and details
- Rabbit polyclonal to RHOH
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-RHOH antibody -
Description
Rabbit polyclonal to RHOH -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide KLH conjugated corresponding to a region from the C terminus of Human RHOH
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Positive control
- Human colon and small intestine tissue. HT-29 cell lysate and A549 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Store undiluted. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 0.88% Sodium chloride, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab118507 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/1000. Predicted molecular weight: 21 kDa.
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IHC-P |
Use a concentration of 5 µg/ml.
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ICC/IF |
1/100 - 1/500.
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Notes |
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WB
1/500 - 1/1000. Predicted molecular weight: 21 kDa. |
IHC-P
Use a concentration of 5 µg/ml. |
ICC/IF
1/100 - 1/500. |
Target
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Function
Negative regulator of hematopoietic progenitor cell proliferation, survival and migration. Critical regulator of thymocyte development and T-cell antigen receptor (TCR) signaling by mediating recruitment and activation of ZAP70. Required for phosphorylation of CD3Z, membrane translocation of ZAP70 and subsequent activation of the ZAP70-mediated pathways. Essential for efficient beta-selection and positive selection by promoting the ZAP70-dependent phosphorylation of the LAT signalosome during pre-TCR and TCR signaling. Crucial for thymocyte maturation during DN3 to DN4 transition and during positive selection. Plays critical roles in mast cell function by facilitating phosphorylation of SYK in Fc epsilon RI-mediated signal transduction. Essential for the phosphorylation of LAT, LCP2, PLCG1 and PLCG2 and for Ca(2+) mobilization in mast cells (By similarity). Binds GTP but lacks intrinsic GTPase activity and is resistant to Rho-specific GTPase-activating proteins. Inhibits the activation of NF-kappa-B by TNF and IKKB and the activation of CRK/p38 by TNF. Inhibits activities of RAC1, RHOA and CDC42. Negatively regulates leukotriene production in neutrophils. -
Tissue specificity
Expressed only in hematopoietic cells. Present at very high levels in the thymus, less abundant in the spleen, and least abundant in the bone marrow. Expressed at a higher level in the TH1 subtype of T-helper cells than in the TH2 subpopulation. Expressed in neutrophils under inflammatory conditions, such as cystic fibrosis, ulcerative colitis and appendicitis. -
Involvement in disease
Note=A chromosomal aberration involving RHOH is found in a non-Hodgkin lymphoma cell line. Translocation t(3;4)(q27;p11) with BCL6. -
Sequence similarities
Belongs to the small GTPase superfamily. Rho family. -
Domain
The region involved in interaction with ZAP70 is a non-canonical immunoreceptor tyrosine-based activation motif (ITAM). -
Post-translational
modificationsPhosphorylated on tyrosine by LCK. Phosphorylated by FYN. Phosphorylation enhances the interactions with ZAP70 and SYK and is critical for its function in thymocyte development. -
Cellular localization
Cytoplasm. Cell membrane. Co-localizes together with ZAP70 in the immunological synapse. - Information by UniProt
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Database links
- Entrez Gene: 399 Human
- Entrez Gene: 74734 Mouse
- Entrez Gene: 305341 Rat
- Omim: 602037 Human
- SwissProt: Q15669 Human
- SwissProt: Q9D3G9 Mouse
- Unigene: 654594 Human
- Unigene: 20323 Mouse
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Alternative names
- ARHH antibody
- GTP binding protein TTF antibody
- GTP-binding protein TTF antibody
see all
Images
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ab118507 at 5 µg/ml staining RHOH in Formalin-Fixed, Paraffin-Embedded Human colon tissue.
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ab118507 at 5 µg/ml staining RHOH in Formalin-Fixed, Paraffin-Embedded Human Small Intestine tissue.
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All lanes : Anti-RHOH antibody (ab118507) at 1/500 dilution
Lane 1 : HT-29 extract without the synthesized peptide.
Lane 2 : HT-29 extract with the synthesized peptide.
Predicted band size: 21 kDa -
Immunofluorescence analysis of A549 cells, using ab118507 at 1/100. The picture on the right is treated with the synthesized peptide.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab118507 has been referenced in 1 publication.
- Kang X et al. miR-3646 promotes vascular inflammation and augments vascular smooth muscle cell proliferation and migration in progression of coronary artery disease by directly targeting RHOH. Int J Clin Exp Pathol 11:5830-5839 (2018). PubMed: 31949669