Overview

  • Product name
    Anti-RHOJ antibody
  • Description
    Rabbit polyclonal to RHOJ
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Saccharomyces cerevisiae, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to a region within internal sequence amino acids 71 - 120 (LGLYDTAGQE DYNQLRPLSY PNTDVFLICF SVVNPASYHN VQEEWVPELK) of Human RHOJ (NP_065714).

  • Positive control
    • 721_B cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab105311 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 24 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Function
    GTP-binding protein with GTPase activity. Elicits the formation of F-actin-rich structures in fibroblasts and is involved in the regulation of cell morphology.
  • Sequence similarities
    Belongs to the small GTPase superfamily. Rho family.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • ARHJ antibody
    • Ras homolog family member J antibody
    • Ras homolog gene family member J antibody
    • RAS like family 7 member B antibody
    • Ras like protein family member 7B antibody
    • Ras-like protein family member 7B antibody
    • RASL 7B antibody
    • RASL7B antibody
    • Rho related GTP binding protein RhoJ antibody
    • Rho-related GTP-binding protein RhoJ antibody
    • RHOI antibody
    • Rhoj antibody
    • RHOJ_HUMAN antibody
    • Tc10 like GTP binding protein TCL antibody
    • TC10 like Rho GTPase antibody
    • Tc10-like GTP-binding protein antibody
    • TC10B antibody
    • TCL antibody
    see all

Images

  • Anti-RHOJ antibody (ab105311) at 1 µg/ml + 721 B cell lysate at 10 µg

    Predicted band size : 24 kDa

References

ab105311 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Sample
Mouse Cell lysate - whole cell (Mouse leukemic stem cells)
Gel Running Conditions
Reduced Non-Denaturing (Native) (4- 20%)
Loading amount
40 µg
Specification
Mouse leukemic stem cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Nov 19 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Hek293 cells transfected with RhoJ-GFP)
Loading amount
20 µg
Specification
Hek293 cells transfected with RhoJ-GFP
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Jul 16 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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