The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 29 kDa. for 2 hours. This antibody has been tested in Western blot against the recombinant peptide used as an immunogen. We have no data on detection of endogenous protein.
Use at an assay dependent dilution.
FunctionPlays a role in the control of the actin cytoskeleton via activation of the JNK pathway.
Tissue specificityHighly expressed in pancreas, placenta, and fetal brain.
Sequence similaritiesBelongs to the small GTPase superfamily. Rho family.
Cellular localizationCell membrane. Endosome membrane. Treatment with TNF activates endosomal but not plasma membrane RHOV.