Overview

  • Product name
    Anti-Rhp51 antibody
  • Description
    Rabbit polyclonal to Rhp51
  • Tested applications
    Suitable for: WB, IP, ICC/IFmore details
  • Species reactivity
    Reacts with: Schizosaccharomyces pombe
  • Immunogen

    Purified recombinant full length protein (Schizosaccharomyces pombe)

  • Positive control
    • Schizosaccharomyces pombe whole cell extracts.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: Whole serum
  • Purity
    Whole antiserum
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab63799 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/5000. Detects a band of approximately 44 kDa (predicted molecular weight: 40 kDa).
IP Use at an assay dependent concentration.
ICC/IF 1/500.

Target

  • Relevance
    Rhp51 is required both for recombination and for the repair of DNA damage caused by X-rays. Binds to single and double stranded DNA, in the presence of magnesium, and exhibits DNA-dependent ATPase activity. Promotes DNA strand annealing and strand exchange via DNA recombinase activity and forms helical nucleoprotein filaments.
  • Cellular localization
    Nuclear
  • Database links
    • Alternative names
      • DNA repair protein rhp51 antibody
      • Rad51 antibody
      • RAD51 homolog antibody
      • Recombinase Rhp51 antibody

    Images

    • ab63799 (1/500) staining Rhp51 in S. pombe cells without or with MMS (0.025%) treatment for 1 hour by ICC/IF (Immunocytochemistry/immunofluorescence).

    • All lanes : Anti-Rhp51 antibody (ab63799) at 1/2000 dilution

      Lane 1 : Molecular weight markers
      Lane 2 : Whole cell extract of Schizosaccharomyces pombe wild-type strain
      Lane 3 : Whole cell extract of Schizosaccharomyces pombe Rad51 deletion strain


      Predicted band size : 40 kDa
      Observed band size : 44 kDa (why is the actual band size different from the predicted?)

    References

    ab63799 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab63799.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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