Overview

  • Product name
    Anti-RIAM antibody [EPR2806]
    See all RIAM primary antibodies
  • Description
    Rabbit monoclonal [EPR2806] to RIAM
  • Tested applications
    Suitable for: WB, IP, ICC, IHC-Pmore details
    Unsuitable for: Flow Cyt
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human RIAM aa 1-100 (N terminal).

  • Positive control
    • Human Lymph node, Fetal brain, Human spleen, Jurkat and HL-60 + retinoic acid cell lysates; Human tonsil tissue
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab92537 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IP
ICC
IHC-P
  • Application notes
    ICC: 1/100 - 1/250.
    IHC-P: 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
    IP: 1/50.
    WB: 1/2000 - 1/10000. Predicted molecular weight: 73 kDa.

    Is unsuitable for Flow Cyt.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance
      RIAM (Rap1-GTP interacting adapter molecule) appears to function in the signal transduction from Ras activation to actin cytoskeletal remodeling via interaction with profilin and VASP. It suppresses insulin-induced promoter activities through AP1 and SRE and also mediates Rap1-induced adhesion.
    • Cellular localization
      Cell Membrane, Cytoplasm and cytoskeleton
    • Database links
    • Alternative names
      • AB1IP antibody
      • Amyloid beta A4 precursor protein binding family B member 1 interacting protein antibody
      • Amyloid beta precursor protein binding family B member 1 antibody
      • APBB1 interacting protein 1 antibody
      • INAG1 antibody
      • OTTHUMP00000019344 antibody
      • PREL 1 antibody
      • PREL1 antibody
      • Proline rich EVH1 ligand 1 antibody
      • Proline rich protein 73 antibody
      • Rap1 GTP interacting adapter molecule antibody
      • RAP1 interacting adaptor molecule antibody
      • RARP 1 antibody
      • RARP1 antibody
      • Retinoic acid responsive proline rich protein 1 antibody
      see all

    Images

    • All lanes : Anti-RIAM antibody [EPR2806] (ab92537) at 1/2000 dilution

      Lane 1 : Human Lymph node lysate
      Lane 2 : Fetal brain lysate
      Lane 3 : Human spleen lysate
      Lane 4 : Jurkat cell lysate
      Lane 5 : HL-60 + retinoic acid cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 73 kDa
    • ab92537, at 1/100 dilution, staining RIAM in paraffin-embedded Human tonsil tissue by Immunohistochemistry.

    References

    This product has been referenced in:
    • Su W  et al. Rap1 and its effector RIAM are required for lymphocyte trafficking. Blood 126:2695-703 (2015). Read more (PubMed: 26324702) »
    • Wynne JP  et al. Rap1-interacting adapter molecule (RIAM) associates with the plasma membrane via a proximity detector. J Cell Biol 199:317-30 (2012). WB . Read more (PubMed: 23045549) »

    See all 2 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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