• Product nameAnti-Ribonuclease 3 antibody
    See all Ribonuclease 3 primary antibodies
  • Description
    Rabbit Polyclonal to Ribonuclease 3
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH, corresponding to a region within C terminal amino acids 123-151 of Human Ribonuclease 3 (NP_002926.2).

  • Positive control
    • NCI-H460 cell line lysates This antibody gave a positive result in IHC in the following FFPE tissue: Human normal kidney.


  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
  • Storage bufferPreservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesab116017 is purified through a protein A column, followed by peptide affinity purification.
  • ClonalityPolyclonal
  • IsotypeIgG


Our Abpromise guarantee covers the use of ab116017 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 18 kDa.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.


  • FunctionCytotoxin and helminthotoxin with low-efficiency ribonuclease activity. Possesses a wide variety of biological activities. Exhibits antibacterial activity.
  • Sequence similaritiesBelongs to the pancreatic ribonuclease family.
  • Cellular localizationCytoplasmic granule. Matrix of eosinophil's large specific granule.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cytotoxic ribonuclease antibody
    • ECP antibody
    • ECP_HUMAN antibody
    • Eosinophil cationic protein antibody
    • OTTHUMP00000164017 antibody
    • Ribonuclease 3 antibody
    • Ribonuclease, RNase A family, 3 antibody
    • RNase 3 antibody
    • RNASE3 antibody
    • RNS3 antibody
    see all

Anti-Ribonuclease 3 antibody images

  • IHC image of Ribonuclease 3 staining in Human normal kidney formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab116017, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.


    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Anti-Ribonuclease 3 antibody (ab116017) at 1/100 dilution + NCI-H460 cell line lysates at 35 µg

    Predicted band size : 18 kDa

References for Anti-Ribonuclease 3 antibody (ab116017)

ab116017 has not yet been referenced specifically in any publications.

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