Overview

  • Product name
    Anti-Ribonuclease 3 antibody
    See all Ribonuclease 3 primary antibodies
  • Description
    Rabbit Polyclonal to Ribonuclease 3
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH, corresponding to a region within C terminal amino acids 123-151 of Human Ribonuclease 3 (NP_002926.2).

  • Positive control
    • NCI-H460 cell line lysates This antibody gave a positive result in IHC in the following FFPE tissue: Human normal kidney.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab116017 is purified through a protein A column, followed by peptide affinity purification.
  • Clonality
    Polyclonal
  • Isotype
    IgG

Applications

Our Abpromise guarantee covers the use of ab116017 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 18 kDa.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Cytotoxin and helminthotoxin with low-efficiency ribonuclease activity. Possesses a wide variety of biological activities. Exhibits antibacterial activity.
  • Sequence similarities
    Belongs to the pancreatic ribonuclease family.
  • Cellular localization
    Cytoplasmic granule. Matrix of eosinophil's large specific granule.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cytotoxic ribonuclease antibody
    • ECP antibody
    • ECP_HUMAN antibody
    • Eosinophil cationic protein antibody
    • OTTHUMP00000164017 antibody
    • Ribonuclease 3 antibody
    • Ribonuclease, RNase A family, 3 antibody
    • RNase 3 antibody
    • RNASE3 antibody
    • RNS3 antibody
    see all

Images

  • IHC image of Ribonuclease 3 staining in Human normal kidney formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab116017, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

     

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Anti-Ribonuclease 3 antibody (ab116017) at 1/100 dilution + NCI-H460 cell line lysates at 35 µg

    Predicted band size : 18 kDa

References

ab116017 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab116017.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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