Anti-Ribonuclease A antibody (ab94417)

Overview

  • Product name
    Anti-Ribonuclease A antibody
    See all Ribonuclease A primary antibodies
  • Description
    Rabbit polyclonal to Ribonuclease A
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Sheep, Rabbit, Goat, Horse, Guinea pig, Cow, Cat, Pig, Chimpanzee, Drosophila melanogaster, African green monkey, Spider monkey
  • Immunogen

    Synthetic peptide corresponding to a region within internal sequence amino acids 108-157 (HITDCRLTNG SRYPNCAYRT SPKERHIIVA CEGSPYVPVH FDASVEDST) of Human Ribonuclease A (NP_002924).

  • Positive control
    • Human fetal kidney lysate IHC-P: Human lung FFPE tissue sections.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab94417 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 18 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

Images

  • IHC image of Ribonuclease A staining in human lung formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab94417, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Anti-Ribonuclease A antibody (ab94417) at 1 µg/ml (in 5% skim milk / PBS buffer) + Human fetal kidney lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 18 kDa
    Gel concentration: 10-20%

References

ab94417 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Sample
Bos taurus Purified protein (mixture of RNase A and B from pancreas)
Gel Running Conditions
Reduced Denaturing (10-20)
Loading amount
20 µg
Treatment
PNGase treatment for 1 hour at 37C
Specification
mixture of RNase A and B from pancreas
Blocking step
ECL prime blocking agent as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 20°C
Username

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Verified customer

Submitted Feb 20 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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