Anti-Ribonuclease H2, subunit A antibody (ab55990)

Overview

  • Product name
    Anti-Ribonuclease H2, subunit A antibody
    See all Ribonuclease H2, subunit A primary antibodies
  • Description
    Rabbit polyclonal to Ribonuclease H2, subunit A
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A region within synthetic peptide: EKEAEDVIWE DSASENQEGL RKITSYFLNE GSQARPRSSH RYFLERGLES, corresponding to amino acids 246-295 of Human Ribonuclease H2, subunit A

  • Positive control
    • Jurkat cell lysate and Human kidney tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab55990 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.25 µg/ml. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.

Target

  • Relevance
    Of the multiple RNases H in mammals, RNase HI is the major enzyme and shows increased activity during DNA replication. It shows more homology to the RNase HII of Escherichia coli.Of the multiple RNases H in mammals, RNase HI is the major enzyme and shows increased activity during DNA replication. It shows more homology to the RNase HII of Escherichia coli.Of the multiple RNases H in mammals, RNase HI is the major enzyme and shows increased activity during DNA replication. It shows more homology to the RNase HII of Escherichia coli.
  • Cellular localization
    Nuclear
  • Database links
  • Alternative names
    • AGS4 antibody
    • Aicardi Goutieres syndrome 4 protein antibody
    • EC 3.1.26.4 antibody
    • JUNB antibody
    • Ribonuclease HI large subunit antibody
    • Ribonuclease HI subunit A antibody
    • RNase H(35) antibody
    • RNase H2 subunit A antibody
    • RNase HI large subunit antibody
    • RNASEH2A antibody
    • RNASEHI antibody
    • RNHIA antibody
    • RNHL antibody
    see all

Images

  • Lane 1 : Molecular Marker
    Lane 2 : Anti-Ribonuclease H2, subunit A antibody (ab55990) at 0.25 µg/ml

    Lane 1 : As above
    Lane 2 : Jurkat cell lysate at 10 µg

    Secondary
    Lane 2 : HRP conjugated anti-Rabbit IgG at 1/50000 dilution


    Predicted band size : 33 kDa
    Observed band size : 33 kDa
    Gel concentration 12%
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling Ribonuclease H2 with ab55990 at 4-8ug/ml. Arrows indicate positively labeled epithelial cells of the renal tubule. Magnification: 400X.

References

ab55990 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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