Anti-Ribonuclease H2, subunit A antibody (ab83943)

Overview

  • Product nameAnti-Ribonuclease H2, subunit A antibody
    See all Ribonuclease H2, subunit A primary antibodies
  • Description
    Rabbit polyclonal to Ribonuclease H2, subunit A
  • Tested applicationsSuitable for: ICC/IF, WB, ELISA, ICCmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (17 amino acids) derived from an internal sequence of Human Ribonuclease H2, subunit A

  • Positive control
    • HeLa cells.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab83943 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 20 µg/ml.
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 33 kDa.
ELISA Use at an assay dependent dilution.
ICC Use a concentration of 2 µg/ml.

Target

  • RelevanceOf the multiple RNases H in mammals, RNase HI is the major enzyme and shows increased activity during DNA replication. It shows more homology to the RNase HII of Escherichia coli.Of the multiple RNases H in mammals, RNase HI is the major enzyme and shows increased activity during DNA replication. It shows more homology to the RNase HII of Escherichia coli.Of the multiple RNases H in mammals, RNase HI is the major enzyme and shows increased activity during DNA replication. It shows more homology to the RNase HII of Escherichia coli.
  • Cellular localizationNuclear
  • Database links
  • Alternative names
    • AGS4 antibody
    • Aicardi Goutieres syndrome 4 protein antibody
    • EC 3.1.26.4 antibody
    • JUNB antibody
    • Ribonuclease HI large subunit antibody
    • Ribonuclease HI subunit A antibody
    • RNase H(35) antibody
    • RNase H2 subunit A antibody
    • RNase HI large subunit antibody
    • RNASEH2A antibody
    • RNASEHI antibody
    • RNHIA antibody
    • RNHL antibody
    see all

Anti-Ribonuclease H2, subunit A antibody images

  • Anti-Ribonuclease H2, subunit A antibody (ab83943) at 1 µg/ml + HeLa cell lysate at 15 µg

    Predicted band size : 33 kDa
    Observed band size : 33 kDa
  • Immunocytochemistry analysis of HeLa cells using ab83943 at 2µg/ml.
  • Immunofluorescence of Rnse H2A in Hela cells using ab83943 at 4.75 ug/ml.

References for Anti-Ribonuclease H2, subunit A antibody (ab83943)

ab83943 has not yet been referenced specifically in any publications.

Product Wall

I am sorry that the antibody has failed to give a signal in western blot. I have processed your request to receive ab56560 as a replacement. Please let me know if there is anything else I can help you with.

Please let me know if increasing the concentration does not prove to be successful. If it does not help, I would be happy to send you ab56560 as a replacement, so that you can try and get a signal in our western blot. I do appreciate your patience in ...

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Thank you for your reply. I am sorry that the replacement antibody that you received failed to work in western blot. I was wondering if with the replacement antibody you tried an overnight incubation of the primary antibody. I only ask, as I remember t...

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I have processed your request to receive a new vial of ab83179 as the original vial failed to give a signal in western blot. If there is anything else I can help you with, please let me know.

Would you like receive another aliquot of ab83179 from a different lot? Or we do have another RNase H1antibody in our catalogue, ab56560 and I was wondering if you would like to try out that antibody?

Thank you for contacting Abcam. I am sorry that you are experiencing problems with ab83179 in western blotting. I have some questions/suggestions about the protocol that you are using. 1 - What concentration of ab83179 are you using? It has been tested...

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Thank you for your inquiry. The lab has confirmed that RNase H2 (ab83943) has been tested on mouse kidney tissue. Please feel free to contact us if you require further assistance.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"