Anti-Ribonuclease Inhibitor antibody (ab77448)

Overview

  • Product nameAnti-Ribonuclease Inhibitor antibody
    See all Ribonuclease Inhibitor primary antibodies
  • Description
    Mouse monoclonal to Ribonuclease Inhibitor
  • Tested applicationsSuitable for: Flow Cyt, WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length Human Ribonuclease Inhibitor protein (AAH11500) with a 26kDa tag.

  • Positive control
    • Ribonuclease Inhibitor transfected 293T cell lysate. Recombinant tagged Human Ribonuclease Inhibitor protein.

Properties

Applications

Our Abpromise guarantee covers the use of ab77448 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 1µg for 106 cells. ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
WB Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).
ELISA Use at an assay dependent concentration.

Target

  • FunctionRibonuclease inhibitor which inhibits RNASE1, RNASE2 and ANG. May play role in redox homeostasis.
  • Sequence similaritiesContains 15 LRR (leucine-rich) repeats.
  • DomainThe LRR domain forms a horseshoe-shaped structure that interacts tightly with target RNAses via a large protein interaction surface on its interior side.
  • Post-translational
    modifications
    The N-terminus is blocked.
    At least 30 of the 32 cysteine residues are in the reduced form.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • AW546468 antibody
    • C80305 antibody
    • MGC18200 antibody
    • MGC4569 antibody
    • MGC54054 antibody
    • OTTHUMP00000147628 antibody
    • OTTHUMP00000229864 antibody
    • OTTHUMP00000229865 antibody
    • OTTHUMP00000229866 antibody
    • OTTHUMP00000229870 antibody
    • OTTHUMP00000229871 antibody
    • OTTHUMP00000229872 antibody
    • Placental ribonuclease inhibitor antibody
    • Placental RNase inhibitor antibody
    • PRI antibody
    • RAI antibody
    • RI antibody
    • Ribonuclease inhibitor antibody
    • Ribonuclease/angiogenin inhibitor 1 antibody
    • Ribonuclease/angiogenin inhibitor antibody
    • RINI_HUMAN antibody
    • RNase inhibitor antibody
    • RNH 1 antibody
    • RNH antibody
    • RNH1 antibody
    • Rnh1 ribonuclease/angiogenin inhibitor 1 antibody
    see all

Anti-Ribonuclease Inhibitor antibody images

  • All lanes : Anti-Ribonuclease Inhibitor antibody (ab77448) at 1 µg/ml

    Lane 1 : Ribonuclease Inhibitor transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution

    Predicted band size : 50 kDa
    Observed band size : 50 kDa
  • Anti-Ribonuclease Inhibitor antibody (ab77448) at 1 µg/ml + Recombinant tagged Human Ribonuclease Inhibitor protein at 0.2 µg

    Secondary
    Goat anti-mouse IgG (H&L)-HRP conjugate at 1/5000 dilution

    Predicted band size : 50 kDa
    Observed band size : 70 kDa (why is the actual band size different from the predicted?)
  • Overlay histogram showing A431 cells stained with ab77448 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab77448, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

References for Anti-Ribonuclease Inhibitor antibody (ab77448)

ab77448 has not yet been referenced specifically in any publications.

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