Overview

  • Product nameAnti-Ribonuclease T2 antibody
    See all Ribonuclease T2 primary antibodies
  • Description
    Rabbit polyclonal to Ribonuclease T2
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to a region within the internal amino acids 108-157 (SRFWKHEWEK HGTCAAQVDA LNSQKKYFGR SLELYRELDL NSVLLKLGIK) of Human Ribonuclease T2 (NP_003721).

  • Positive control
    • 293T cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab86165 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 29 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P 1/100.

Target

  • Tissue specificityUbiquitous. Higher expression levels observed in the temporal lobe and fetal brain.
  • Involvement in diseaseDefects in RNASET2 are the cause of leukoencephalopathy cystic without megalencephaly (LCWM) [MIM:612951]. An infantile-onset syndrome of cerebral leukoencephalopathy. Affected newborns develop microcephaly and neurologic abnormalities including psychomotor impairment, seizures and sensorineural hearing impairment. The brain shows multifocal white matter lesions, anterior temporal lobe subcortical cysts, pericystic abnormal myelination, ventriculomegaly and intracranial calcifications.
  • Sequence similaritiesBelongs to the RNase T2 family.
  • Cellular localizationSecreted. Subcellular fractionation of transfected ovarian cancer cells reveals full-length RNASET2 in the endoplasmic reticulum fraction and the 2 smaller RNASET2 proteolytic products in the lysosome fraction.
  • Information by UniProt
  • Database links
  • Alternative names
    • bA514O12.3 antibody
    • Ribonuclease 6 antibody
    • Ribonuclease T2 antibody
    • RNASE6PL antibody
    • Rnaset2 antibody
    • RNT2_HUMAN antibody
    • RP11 514O12.3 antibody
    see all

Anti-Ribonuclease T2 antibody images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung tissue labelling Ribonuclease T2 with ab86165 at 1/100. A Cy3-conjugated donkey anti-rabbit IgG (1/200) was used as the secondary antibody. Positive staining seen in the cytoplasm of alveolar cells. type I and II. Magnification: 20X. Exposure time: 0.5 - 2.0 seconds. Left - DAPI. Middle - Ribonuclease T2. Right - Merge.
  • Anti-Ribonuclease T2 antibody (ab86165) at 1 µg/ml (in 5% skim milk / PBS buffer) + 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 29 kDa
    Observed band size : 35 kDa (why is the actual band size different from the predicted?)
    Additional bands at : >90 kDa. We are unsure as to the identity of these extra bands.
    Gel concentration: 12%

References for Anti-Ribonuclease T2 antibody (ab86165)

ab86165 has not yet been referenced specifically in any publications.

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