Anti-Ribosomal protein L26 antibody - Aminoterminal end (ab59567)

Overview

  • Product nameAnti-Ribosomal protein L26 antibody - Aminoterminal end
    See all Ribosomal protein L26 primary antibodies
  • Description
    Rabbit polyclonal to Ribosomal protein L26 - Aminoterminal end
  • SpecificityThis antibody specifically recognizes human Ribosomal Protein L26.
  • Tested applicationsSuitable for: ICC/IF, WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow, Xenopus laevis, Cynomolgus Monkey
  • Immunogen

    Synthetic peptide:

    MKFNPFVTSDRSKNRKR

    conjugated to KLH via a C-terminal added cysteine residue, corresponding to N terminal amino acids 1-17 of Human Ribosomal protein L26

  • Positive control
    • HEK-293 T cell extracts and paraformadehyde-fixed HeLa cells.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab59567 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 - 10 µg/ml.
WB Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 17 kDa (predicted molecular weight: 17 kDa).

Staining of the ribosomal protein L26 band in immunoblotting is specifically inhibited by the immunizing peptide.

IP Use a concentration of 5 - 10 µg/ml.

Target

Anti-Ribosomal protein L26 antibody - Aminoterminal end images

  • Cytoplasmic localization of Ribosomal Protein L26 in HeLa cells stained with ab59567 at a 5µg/mL concentration (A), and DAPI (B). The antibody was developed with Goat Anti-Rabbit IgG, Cy3 conjugated.
  • All lanes : Anti-Ribosomal protein L26 antibody - Aminoterminal end (ab59567) at 0.25 µg/ml

    Lane 1 : 293T
    Lane 2 : HeLa
    Lane 3 : U2OS
    Lane 4 : MCF7
    Lane 5 : K562
    Lane 6 : A431
    Lane 7 : Jurkat
    Lane 8 : HepG2
    Lane 9 : NIH3T3
    Lane 10 : PC12
    Lane 11 : RAT1

    Secondary
    Goat Anti-rabbit IgG, Peroxidase conjugate

    Predicted band size : 17 kDa
    Observed band size : 17 kDa

References for Anti-Ribosomal protein L26 antibody - Aminoterminal end (ab59567)

This product has been referenced in:
  • Fathinajafabadi A  et al. CERKL, a retinal disease gene, encodes an mRNA-binding protein that localizes in compact and untranslated mRNPs associated with microtubules. PLoS One 9:e87898 (2014). WB ; Human . Read more (PubMed: 24498393) »
  • Leitman J  et al. Herp coordinates compartmentalization and recruitment of HRD1 and misfolded proteins for ERAD. Mol Biol Cell 25:1050-60 (2014). Human . Read more (PubMed: 24478453) »

See all 6 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Blocking step PBS-1% ovalbumin as blocking agent for 20 minute(s) · Concentration: 1.0% · Temperature: 22°C
Sample Salmo salar Cell (testicular)
Specification testicular
Permeabilization Yes - methyl methacrylate/Butyl methacrylate
Fixative glutaraldehyde-formaldehyde
Username

Abcam user community

Verified customer

Submitted Mar 25 2015

Application Western blot
Sample Saccharomyces cerevisiae Cell lysate - other (Ribosome enriched sample)
Loading amount 20 µg
Specification Ribosome enriched sample
Gel Running Conditions Reduced Denaturing (4-12% Bis-Tris)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Dr. Joseph Moore IV

Verified customer

Submitted Dec 21 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Hela)
Loading amount 20 µg
Specification Hela
Gel Running Conditions Reduced Denaturing
Blocking step PBST as blocking agent for 15 minute(s) · Concentration: 0.1% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Nov 18 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"