Overview

  • Product name
    Anti-Ribosomal Protein S29 antibody
  • Description
    Rabbit polyclonal to Ribosomal Protein S29
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig, Zebrafish
  • Immunogen

    Synthetic peptide: WSHPRKFGQG SRSCRVCSNR HGLIRKYGLN MCRQCFRQYA KDIGFIKLD, corresponding to amino acids 8-56 of Human Ribosomal Protein S29

  • Positive control
    • Jurkat cell lysate

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab56224 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 7 kDa (predicted molecular weight: 7 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/312500.

Target

Anti-Ribosomal Protein S29 antibody images

  • All lanes : Anti-Ribosomal Protein S29 antibody (ab56224) at 1.25 µg/ml

    Lane 1 : Marker
    Lane 2 : Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 7 kDa
    Observed band size : 7 kDa

References for Anti-Ribosomal Protein S29 antibody (ab56224)

This product has been referenced in:
  • Li C  et al. Proteomic Analysis of Liver Proteins in a Rat Model of Chronic Restraint Stress-Induced Depression. Biomed Res Int 2017:7508316 (2017). Read more (PubMed: 28293639) »
  • Iavello A  et al. Role of Alix in miRNA packaging during extracellular vesicle biogenesis. Int J Mol Med 37:958-66 (2016). WB . Read more (PubMed: 26935291) »

See all 3 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Prostate)
Specification
Prostate
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Novocastra pH 6 retrieval buffer
Permeabilization
No
Blocking step
H2O2 as blocking agent for 10 minute(s) · Concentration: 0.3% · Temperature: 22°C
Username

Dr. Aamir Ahmed

Verified customer

Submitted Oct 05 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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