Overview

  • Product nameAnti-ribosomal RNA 5.8s antibody [Y10b]
    See all ribosomal RNA 5.8s primary antibodies
  • Description
    Mouse monoclonal [Y10b] to ribosomal RNA 5.8s
  • SpecificityThis antibody is specific for ribosomal RNA. There is no cross-reactivity with other RNAs.
  • Tested applicationsSuitable for: IP, ICC/IFmore details
  • Species reactivity
    This antibody reacts with all eukaryotes.
  • Immunogen

    The whole 5.8S ribosomal RNA.

  • EpitopeThis antibody is useful for recognizing RNA epitopes on both the large and small subunit.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.05% Sodium Azide
  • Concentration information loading...
  • PurityProtein A purified
  • Purification notesProtein A purified mouse ascites.
  • ClonalityMonoclonal
  • Clone numberY10b
  • IsotypeIgG3
  • Light chain typekappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab37144 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent dilution.
ICC/IF Use at an assay dependent dilution.

Target

  • RelevanceThe inhibition of protein synthesis by specific anti 5.8S rRNA oligonucleotides have suggested that this RNA plays an important role in eukaryotic ribosome function, specifically ribosomal translocation. Mutations in the 5.8S rRNA can inhibit cell growth and compromise protein synthesis in vitro.
  • Cellular localizationCytoplasmic and Nuclear

References for Anti-ribosomal RNA 5.8s antibody [Y10b] (ab37144)

This product has been referenced in:
  • Zhu CH  et al. SGNP: an essential Stress Granule/Nucleolar Protein potentially involved in 5.8s rRNA processing/transport. PLoS ONE 3:e3716 (2008). ICC/IF ; Human . Read more (PubMed: 19005571) »
  • Thomas MG  et al. Staufen recruitment into stress granules does not affect early mRNA transport in oligodendrocytes. Mol Biol Cell 16:405-20 (2005). Read more (PubMed: 15525674) »

See all 14 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Sample Human Cell (Lymphoblast)
Specification Lymphoblast
Permeabilization Yes - 0.1% TX-100 for 10 min.
Fixative Paraformaldehyde
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Submitted Mar 26 2014

Application Immunocytochemistry/ Immunofluorescence
Sample Rat Cell (brain)
Specification brain
Fixative paraformaldehyde + glutaraldehyde
Permeabilization No
Blocking step Serum as blocking agent for 10 minute(s) · Concentration: 10% · Temperature: 22°C
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Submitted Dec 09 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"