The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesPeptide ELISA: Antibody detection limit dilution 1:32,000.
WB: Use at a concentration of 1 - 3 µg/ml. Predicted molecular weight: 60 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
RelevanceRIC8 is a Guanine nucleotide exchange factor (GEF), which can activate some, but not all, G-alpha proteins by exchanging bound GDP for free GTP.
The Caenorhabditis elegans protein Ric-8 has two mammalian homologs that have been identified : Ric-8A (Ric-8/synembryn) and Ric-8B. Synembryns are proteins that positively regulate synaptic transmission.