Overview

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline. pH 7.3
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab24383 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
WB
  • Application notesPeptide ELISA: Antibody detection limit dilution 1:32,000.
    WB: Use at a concentration of 1 - 3 µg/ml. Predicted molecular weight: 60 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceRIC8 is a Guanine nucleotide exchange factor (GEF), which can activate some, but not all, G-alpha proteins by exchanging bound GDP for free GTP. The Caenorhabditis elegans protein Ric-8 has two mammalian homologs that have been identified : Ric-8A (Ric-8/synembryn) and Ric-8B. Synembryns are proteins that positively regulate synaptic transmission.
    • Cellular localizationCytoplasmic
    • Database links
    • Alternative names
      • Brain synembrin antibody
      • FLJ10620 antibody
      • hSyn antibody
      • MGC104517 antibody
      • MGC131931 antibody
      • MGC148073 antibody
      • MGC148074 antibody
      • MGC39476 antibody
      • Protein Ric 8A antibody
      • Protein Ric 8B antibody
      • Protein Ric8A antibody
      • Protein Ric8B antibody
      • Resistance to inhibitors of cholinesterase 8 homolog A (C. elegans) antibody
      • Resistance to inhibitors of cholinesterase 8 homolog A antibody
      • Resistance to inhibitors of cholinesterase 8 homolog B (C. elegans) antibody
      • Resistance to inhibitors of cholinesterase 8 homolog B antibody
      • RIC 8 antibody
      • RIC 8A antibody
      • RIC 8B antibody
      • RIC8A antibody
      • RIC8B antibody
      • Synembryn A antibody
      • Synembryn antibody
      • Synembryn B antibody
      • SynembrynA antibody
      • SynembrynB antibody
      see all

    Anti-RIC8 antibody images

    References for Anti-RIC8 antibody (ab24383)

    ab24383 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (HeLa, Jurkat)
    Loading amount 100 µg
    Specification HeLa, Jurkat
    Gel Running Conditions Reduced Denaturing (10%)
    Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 18°C
    Username

    Mrs. Merly Saare

    Verified customer

    Submitted Jan 15 2010

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"