Anti-RIC8A antibody (ab194941)
Key features and details
- Rabbit polyclonal to RIC8A
- Suitable for: IP, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-RIC8A antibody
See all RIC8A primary antibodies -
Description
Rabbit polyclonal to RIC8A -
Host species
Rabbit -
Tested applications
Suitable for: IP, WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Sheep, Rabbit, Horse, Cow, Dog, Pig, Chimpanzee, Cynomolgus monkey, Rhesus monkey, Gorilla, Orangutan -
Immunogen
Recombinant fragment within Human RIC8A aa 400-500. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: Q9NPQ8 -
Positive control
- Whole cell lysate from HeLa, 293T, Jurkat, mouse TCMK-1 and mouse NIH 3T3 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab194941 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IP |
Use at 2-10 µg/mg of lysate.
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WB |
1/2000 - 1/10000. Predicted molecular weight: 60 kDa.
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Notes |
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IP
Use at 2-10 µg/mg of lysate. |
WB
1/2000 - 1/10000. Predicted molecular weight: 60 kDa. |
Target
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Function
Guanine nucleotide exchange factor (GEF), which can activate some, but not all, G-alpha proteins. Able to activate GNAI1, GNAO1 and GNAQ, but not GNAS by exchanging bound GDP for free GTP. Involved in regulation of microtubule pulling forces during mitotic movement of chromosomes by stimulating G(i)-alpha protein, possibly leading to release G(i)-alpha-GTP and NuMA proteins from the NuMA-GPSM2-G(i)-alpha-GDP complex (By similarity). Also acts as an activator for G(q)-alpha (GNAQ) protein by enhancing the G(q)-coupled receptor-mediated ERK activation. -
Sequence similarities
Belongs to the synembryn family. -
Cellular localization
Cytoplasm. Cell membrane. Colocalizes with RIC8A in CA2 hippocampal neurons. Colocalizes with GNAI1 and RGS14 at the plasma membrane (By similarity). - Information by UniProt
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Database links
- Entrez Gene: 60626 Human
- Entrez Gene: 101489 Mouse
- Entrez Gene: 293614 Rat
- Omim: 609146 Human
- SwissProt: Q5E9J8 Cow
- SwissProt: Q9NPQ8 Human
- SwissProt: Q3TIR3 Mouse
- SwissProt: Q5R8F5 Orangutan
see all -
Alternative names
- MGC104517 antibody
- MGC131931 antibody
- MGC148073 antibody
see all
Images
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All lanes : Anti-RIC8A antibody (ab194941) at 0.1 µg/ml
Lane 1 : Whole cell lysate from HeLa
Lane 2 : Whole cell lysate from 293T
Lane 3 : Whole cell lysate from Jurkat
Lane 4 : Whole cell lysate from TCMK-1
Lane 5 : Whole cell lysate from NIH3T3
Lysates/proteins at 50 µg per lane.
Predicted band size: 60 kDa -
Detection of Human RIC8A by Western Blot of Immunoprecipitate.
Lane 1: Whole cell lysate from Jurkat cells (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) prepared using NETN lysis buffer. ab194941 was used for IP at 6 μg per reaction, and at 1 μg/ml for blotting the immunoprecipitate.
Lane 2: Control IgG.
Detection: Chemiluminescence with an exposure time of 30 seconds.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab194941 has not yet been referenced specifically in any publications.