The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesELISA: <1/10000.
WB: >1/500. Detects a band of approximately 92 kDa (predicted molecular weight: 89 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
RelevanceRICH2, Rho GTPase-activating protein interacting with CIP homologue protein 2, is a member Rho GTPase activating protein RICH family. This family of protein is one of the major regulators of Rho GTPases in all eukaryotes. In addition to their role as specific negative regulators of Rho protein signaling pathways, they are crucial in the differentiation, growth and organization of cell cytoskeleton, neuronal development and synaptic functions. RICH2 is located at mouse chromosome 11B3 and as a GTPase activator for the Rho-type GTPases, it converts them to an inactive GDP-bound state. Recent reports have identified RICH2 as a GTPase activator for Cdc42 and Rac1. RICH2 and different isoforms of RICH1 share two conserved domains, an N-terminal BAR domain and Rho/Rac/cdc42 GAP domain. The BAR domain of RICH2 binds membranes and is capable of inducing membrane tabulation. As a member of RICH family
of proteins, RICH2 plays a crucial role in linking CD317/tetherin to the apical actin network.
Knocking down the expression of RICH2 in cells usually results in loss of apical actin network. These cells exhibited similar characteristics that were seen in the CD317 knockdown cells. These characteristics include subsequent loss of apical microvilli, increase in actin bundles at the basal surface, reduction in cell height without any loss of tight junctions or transepithelial resistance or any change in the distribution of apical and basolateral marker proteins. Overexpression of RICH2 leads to a loss of Ras-induced membrane ruffles.